mitoNGS / MToolBox

A bioinformatics pipeline to analyze mtDNA from NGS data
http://sourceforge.net/projects/mtoolbox/?source=navbar
GNU General Public License v3.0
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trouble while trying test example #113

Closed snackens closed 4 months ago

snackens commented 5 months ago

Hi, Thank you for this good tools.

I tried to run with test example, but something wrong.

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EXECUTING READ MAPPING WITH MAPEXOME...

mapExome for sample SRR043366, files found: SRR043366.R1.fastq.gz SRR043366.R2.fastq.gz Mapping onto mtDNA... -D -D /usr/local/share/gmapdb --gunzip -d chrM -A sam --nofails --pairmax-dna=500 --query-unk-mismatch=1 --read-group-id=sample --read-group-name=sample --read-group-library=sample --read-group-platform=sample -n 1 -Q -O -t 8 SRR043366.R1.fastq.gz SRR043366.R2.fastq.gz > /home/user/mtDNA/test/out//OUT_SRR043366/outmt.sam 2> /home/user/mtDNA/test/out//OUT_SRR043366/logmt.txt sh: 0: Illegal option -D Extracting FASTQ from SAM... Traceback (most recent call last): File "/home/user/MToolBox/mapExome.py", line 96, in f=open(mtoutsam) IOError: [Errno 2] No such file or directory: '/home/user/mtDNA/test/out//OUT_SRR043366/outmt.sam'

== I think -D -D /usr.... is wrong. Is this install problem?

Best regards,