Open AlenCarral opened 12 months ago
I think it can be solved by multiplying a negative sign to the ctf function or the image in the fourier space.
I also encountered the same situation. Did you resolve the problem?
Same situation, and after multiplying -1 to the reconstructed volume, I get a seemingly correct result.
I strictly followed the steps provided in the README file. However, I encountered an issue where the reconstruction protein model appears to have a hollow structure, whether w/wo gt-pose:
left: wo gt-pose right with gt-pose
ps: I have already validated the input data by running cryodrgn and cryodrgn2 using exact the same set of data, they produced reasonable structures:![image](https://github.com/ml-struct-bio/cryofire/assets/132803488/7548acf3-a505-410c-8d10-5889c12854c0)
Has anyone encountered a similar situation before?Or can someone provide some ideas or suggestions?