Closed sarakn97 closed 1 year ago
I am trying to run the following script so I can use the h5ad file in seurat. The Convert() function successfully ran and now my RStudio crashes every-time that I run the LoadH5Seurat() command.
# scrna analysis h5ad t-cells library(Seurat) library(SeuratDisk) library(devtools) library(SeuratData) Convert("./t-cells.h5ad", dest = "h5seurat", overwrite = TRUE) Tcells <- LoadH5Seurat("./t-cells.h5seurat") Tcells@meta.data
It begins to run and spits out :
Validating h5seurat file Initializing RNA with data
.... Then it Crashes. "RStudio Session Aborted." RStudio Session Info:
R version 4.1.2 (2021-11-01) Platform: x86_64-pc-linux-gnu (64-bit) Running under: Ubuntu 20.04.3 LTS Matrix products: default BLAS: /usr/lib/x86_64-linux-gnu/blas/libblas.so.3.9.0 LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.9.0 locale: [1] LC_CTYPE=C.UTF-8 LC_NUMERIC=C [3] LC_TIME=C.UTF-8 LC_COLLATE=C.UTF-8 [5] LC_MONETARY=C.UTF-8 LC_MESSAGES=C.UTF-8 [7] LC_PAPER=C.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=C.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] stats graphics grDevices utils datasets methods [7] base other attached packages: [1] SeuratData_0.2.2 devtools_2.4.4 usethis_2.1.6 [4] SeuratDisk_0.0.0.9020 SeuratObject_4.1.3 Seurat_4.3.0 loaded via a namespace (and not attached): [1] Rtsne_0.16 colorspace_2.0-3 [3] deldir_1.0-6 ellipsis_0.3.2 [5] ggridges_0.5.4 fs_1.5.2 [7] rstudioapi_0.13 spatstat.data_3.0-0 [9] leiden_0.4.3 listenv_0.8.0 [11] remotes_2.4.2 ggrepel_0.9.2 [13] bit64_4.0.5 fansi_1.0.3 [15] codetools_0.2-18 splines_4.1.2 [17] cachem_1.0.6 pkgload_1.3.0 [19] polyclip_1.10-4 jsonlite_1.8.3 [21] ica_1.0-3 cluster_2.1.2 [23] png_0.1-8 uwot_0.1.14 [25] shiny_1.7.3 sctransform_0.3.5 [27] spatstat.sparse_3.0-0 compiler_4.1.2 [29] httr_1.4.4 assertthat_0.2.1 [31] Matrix_1.5-3 fastmap_1.1.0 [33] lazyeval_0.2.2 cli_3.4.1 [35] later_1.3.0 prettyunits_1.1.1 [37] htmltools_0.5.3 tools_4.1.2 [39] igraph_1.3.5 gtable_0.3.1 [41] glue_1.6.2 RANN_2.6.1 [43] reshape2_1.4.4 dplyr_1.0.10 [45] rappdirs_0.3.3 Rcpp_1.0.9 [47] scattermore_0.8 vctrs_0.5.1 [49] spatstat.explore_3.0-5 nlme_3.1-153 [51] progressr_0.11.0 lmtest_0.9-40 [53] spatstat.random_3.0-1 stringr_1.4.1 [55] ps_1.7.1 globals_0.16.2 [57] mime_0.12 miniUI_0.1.1.1 [59] lifecycle_1.0.3 irlba_2.3.5.1 [61] goftest_1.2-3 future_1.29.0 [63] MASS_7.3-54 zoo_1.8-11 [65] scales_1.2.1 promises_1.2.0.1 [67] spatstat.utils_3.0-1 parallel_4.1.2 [69] RColorBrewer_1.1-3 memoise_2.0.1 [71] reticulate_1.26 pbapply_1.6-0 [73] gridExtra_2.3 ggplot2_3.4.0 [75] stringi_1.7.8 pkgbuild_1.3.1 [77] rlang_1.0.6 pkgconfig_2.0.3 [79] matrixStats_0.63.0 lattice_0.20-45 [81] ROCR_1.0-11 purrr_0.3.5 [83] tensor_1.5 patchwork_1.1.2 [85] htmlwidgets_1.5.4 processx_3.7.0 [87] cowplot_1.1.1 bit_4.0.5 [89] tidyselect_1.2.0 parallelly_1.32.1 [91] RcppAnnoy_0.0.20 plyr_1.8.8 [93] magrittr_2.0.3 R6_2.5.1 [95] profvis_0.3.7 generics_0.1.3 [97] DBI_1.1.3 pillar_1.8.1 [99] withr_2.5.0 fitdistrplus_1.1-8 [101] survival_3.2-13 abind_1.4-5 [103] sp_1.5-1 tibble_3.1.8 [105] future.apply_1.10.0 crayon_1.5.2 [107] hdf5r_1.3.7 KernSmooth_2.23-20 [109] utf8_1.2.2 spatstat.geom_3.0-3 [111] plotly_4.10.1 urlchecker_1.0.1 [113] grid_4.1.2 data.table_1.14.6 [115] callr_3.7.1 digest_0.6.30 [117] xtable_1.8-4 tidyr_1.2.1 [119] httpuv_1.6.6 munsell_0.5.0 [121] viridisLite_0.4.1 sessioninfo_1.2.2
I set 32GB as the memory for this RStudio Session and before running ReadH5Seurat() I have only used 20%.
Nevermind, Its a very large file so I was running out of memory and had to allot more space.
I am trying to run the following script so I can use the h5ad file in seurat. The Convert() function successfully ran and now my RStudio crashes every-time that I run the LoadH5Seurat() command.
It begins to run and spits out :
.... Then it Crashes. "RStudio Session Aborted." RStudio Session Info:
I set 32GB as the memory for this RStudio Session and before running ReadH5Seurat() I have only used 20%.