Open iimog opened 6 years ago
Possible problem. We want to search DNA so in HMMER >=3.1
there are special tools for that nhmmer
and nhmmscan
. Those have the following purpose (see manual):
But what we would need is the DNA equivalent to:
However this does not exist. First experiments indicate that both nhmmer
and nhmmscan
could work for our purpose but there might be problems with either large sequence databases or long query sequences.
Important: hmmsearch
only searches the forward strand (as it would with proteins). nhmmer
also considers the reverse strand. So either switch over to that or run hmmsearch on both the normal sequences and the rc of all sequences.
Currently only dispr is included for filtering and orientation. However HMMs would make sense as well. Especially, as we have good HMMs for the borders of the ITS2.