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Synthetic Lethal Screen of an EGFR-Centered Network to Improve Targeted Therapies #162

Closed pnrobinson closed 4 years ago

pnrobinson commented 4 years ago

Astsaturov and colleagues identified SLs in human by combining both computational and experimental approaches [18].They first combined pathway maps, protein-protein interactions, gene expression data and human orthologs of Drosophila Egfr genetic interaction partners, to predict 2689 SL candidates of EGFR. They then selected 683 candidates for RNAi screening, by their appearance in at least two of these information sources or by prior biological knowledge, which resulted in 61 SLs.

https://stke.sciencemag.org/content/3/140/ra67

pnrobinson commented 4 years ago

done

pnrobinson commented 4 years ago

Analysis based on the Chou-Talalay coefficient of interaction showed that the small-molecule AURKA inhibitor PHA-680632 (29) synergized with erlotinib in reducing cell viability of both A431 and HCT116 cells (Fig. 6B). In HCT116 cells, we found strong synergy (coefficient of interaction values <0.5) between cetuximab and either PHA-680632 or another AURKA inhibitor, C1368

pnrobinson commented 4 years ago

https://pubmed.ncbi.nlm.nih.gov/20858866/ done