moontreegy / scseq_data_formatting

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31413257_Human_Blood #62

Closed liuyifang closed 11 months ago

liuyifang commented 3 years ago

PMID: 31413257

Species: Human

Tissue: Blood

Paper title: A general approach for detecting expressed mutations in AML cells using single cell RNA-sequencing

Paper link: https://www-nature-com.ezp-prod1.hul.harvard.edu/articles/s41467-019-11591-1

dbGaP link: https://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/projects/gap/cgi-bin/study.cgi?study_id=phs000159.v8.p4

Note:

Get Seurat object from https://zenodo.org/record/3345981#.X3soxJNKhtY

5 patient datasets with a combined one, select up to top 100 markers for each cluster (order by avg_logFC, p_val < 0.05), only calculate markers for cluster contains not less than 3 cells.

liuyifang commented 3 years ago

@drsc-user Aram,

Below is the data for 31413257_Human_Blood: /n/groups/flyrnai/yifang/Projects/With/Yue_Aram/scseq_data_formatting/processing_data/31413257_Human_Blood/results/