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mothur / mothur.github.io

wiki for the mothur software package
https://mothur.github.io
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pcr.seqs #45

Closed aganve closed 9 months ago

aganve commented 4 years ago

Hello, Can I use the pcr.seqs command to isolate my PCR insert from the plasmid vector? I see that it has the option to trim up to a certain point and trim from a certain point. Can I use pcr.seqs to accomplish this? And will it output my trimmed fasta sequences?

Thanks.

pschloss commented 4 years ago

Hi - you should be able to do that, but there are probably tools better designed for working with plasmids and cloning products.

Pat

On Oct 15, 2020, at 3:29 PM, aganve notifications@github.com wrote:

Hello, Can I use the pcr.seqs command to isolate my PCR insert from the plasmid vector? I see that it has the option to trim up to a certain point and trim from a certain point. Can I use pcr.seqs to accomplish this? And will it output my trimmed fasta sequences?

Thanks.

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