motu-tool / mOTUs

motus - a tool for marker gene-based OTU (mOTU) profiling
GNU General Public License v3.0
144 stars 24 forks source link

no abundance #106

Closed ucassee closed 1 year ago

ucassee commented 1 year ago

Hi,

I use the following command to run mOTUs, but it seems that no reads are mapped to the reference database.

motus profile -s ANT29P4-06_1.fastq -n ANT29P4-06 -t 40 -q -o ANT29P4-06_taxonomy_profile.txt

My sample is from Antarctic Pole. The length of my reads is 150bp. I attached the output file. I was wondering whether this result is normal. ANT29P4-06_taxonomy_profile.txt

Thanks,

AlessioMilanese commented 1 year ago

Hi,

You can see with the command:

cat ANT29P4-06_taxonomy_profile.txt | grep -v "0.0000000000"

the species that are not zero. You have 52 ref-mOTUs, 1 meta-mOTU and 56 ext-mOTUs, plus the unassigned.

ucassee commented 1 year ago

Hi Alessio,

I get it. Thanks for your timely response. I will use mOTUs to analyze more samples.

Best, Yingli

AlessioMilanese commented 1 year ago

Nice! Thanks for your interest in mOTUs. Let us know if everything works out.