Hi Mitchell,
I was doing comparison of the centromere sequence of two species. When I set the parameters to “window: 500, cooler_window: 600, mm_f: 1000” the program went well. But the monomer sequence of my centromere was ~36bp, so I want to change the window size to a smaller one. After the parameters were set to "window: 32, cooler_window: 100, mm_f: 1000", I got the following errors:
Error in rule aln_prep:
jobid: 8
input: results/Agig_CEN5_Afim_cen4.32.fasta
output: temp/Agig_CEN5_Afim_cen4.32.1000.ref_0.fasta.mmi
log: logs/aln_prep.Agig_CEN5_Afim_cen4.32.1000.ref_0.log (check log file(s) for error details)
conda-env: /Users/hippo/Documents/JH_StainedGlass_analysis/Agig_CEN5_Afimcen4/.snakemake/conda/18f28c24ae197bbcc7e830a560e33b11
shell:
minimap2 -f 1000 -s 6 -a --no-pairing -k21 --sr -A2 -B8 -O12,32 -E2,1 -r100 -g100 --heap-sort=yes -X -w11 -e0 -d temp/Agig_CEN5_Afim_cen4.32.1000.ref_0.fasta.mmi results/Agig_CEN5_Afim_cen4.32.fasta 2> logs/aln_prep.Agig_CEN5_Afim_cen4.32.1000.ref_0.log
(one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
Shutting down, this might take some time.
Exiting because a job execution failed. Look above for error message
Complete log: .snakemake/log/2023-08-04T113517.379978.snakemake.log
And the logs/aln_prep.Agig_CEN5_Afim_cen4.32.1000.ref_0.log file had only one line:
[ERROR] unknown option in "-w11"
I don't know if I got this error when the smaller window size was set up, and how to solve this, could you help me out? Thanks!
Hi Mitchell, I was doing comparison of the centromere sequence of two species. When I set the parameters to “window: 500, cooler_window: 600, mm_f: 1000” the program went well. But the monomer sequence of my centromere was ~36bp, so I want to change the window size to a smaller one. After the parameters were set to "window: 32, cooler_window: 100, mm_f: 1000", I got the following errors:
Error in rule aln_prep: jobid: 8 input: results/Agig_CEN5_Afim_cen4.32.fasta output: temp/Agig_CEN5_Afim_cen4.32.1000.ref_0.fasta.mmi log: logs/aln_prep.Agig_CEN5_Afim_cen4.32.1000.ref_0.log (check log file(s) for error details) conda-env: /Users/hippo/Documents/JH_StainedGlass_analysis/Agig_CEN5_Afimcen4/.snakemake/conda/18f28c24ae197bbcc7e830a560e33b11 shell: minimap2 -f 1000 -s 6 -a --no-pairing -k21 --sr -A2 -B8 -O12,32 -E2,1 -r100 -g100 --heap-sort=yes -X -w11 -e0 -d temp/Agig_CEN5_Afim_cen4.32.1000.ref_0.fasta.mmi results/Agig_CEN5_Afim_cen4.32.fasta 2> logs/aln_prep.Agig_CEN5_Afim_cen4.32.1000.ref_0.log (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) Shutting down, this might take some time. Exiting because a job execution failed. Look above for error message Complete log: .snakemake/log/2023-08-04T113517.379978.snakemake.log
And the logs/aln_prep.Agig_CEN5_Afim_cen4.32.1000.ref_0.log file had only one line: [ERROR] unknown option in "-w11"