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mrymsadeghi / AMBIA

MIT License
30 stars 4 forks source link

blevel image not complete #6

Open meripuv opened 9 months ago

meripuv commented 9 months ago

Dear AMBIA developer(s) and fellow AMBIA users Thanks a lot for creating this tool! I ran into a very weird issue: with some of my images (czi format), only a subset of the blevel image is created after successful slice detection (the alevel image is correct). I attached an example image below. I already tried to reduce the size of the original image, which sometimes helped, but not always. Any suggestion to solve this problem would be highly appreciated!

blevel_eq

mrymsadeghi commented 2 months ago

Could you maybe share your slide so we can check?

meripuv commented 2 months ago

slides can be downloaded here (within 7 days from now): https://www.transfernow.net/dl/20241001hlMiP9tt I put two slides: 98-L_slide1-slice1_abetaCy5_raw-Extended Depth of Focus-02-Sample down-01.czi -> this one works fine 98-L_slide1-slice5_abetaCy5_raw-Extended Depth of Focus-04-Sample down-02.czi -> this one gives a weird b-level image