Open Jay-Leung opened 2 years ago
In the prior thread cyriac said "The best option for now is to use the official GRCh38 reference FASTA from NCBI". @ckandoth do you mind providing the path to that fasta? Like Jay I have tried downloading a couple different fastas I found online after googling "official GRCh38 reference FASTA from NCBI," but all result in the same 'chr' error
I also have same problem in chr10...
Same here, I'm getting
[faidx] Failed to fetch sequence in chr10
with all downloaded fastas, either NCBI or VEP or USCS
Has someone found a solution??
Hello!
I overcame this issue by adding chr* definitions to Homo_sapiens.GRCh38.dna.toplevel.fa.gz.fai :
1 248956422 56 60 61
2 242193529 253105808 60 61
3 198295559 499335952 60 61
4 190214555 700936493 60 61
5 181538259 894321347 60 61
6 170805979 1078885300 60 61
7 159345973 1252538102 60 61
8 145138636 1414539898 60 61
9 138394717 1562097568 60 61
10 133797422 1702798922 60 61
11 135086622 1838826360 60 61
12 133275309 1976164484 60 61
13 114364328 2111661107 60 61
14 107043718 2227931566 60 61
15 101991189 2336759404 60 61
16 90338345 2440450504 60 61
17 83257441 2532294546 60 61
18 80373285 2616939669 60 61
19 58617616 2698652566 60 61
20 64444167 2758247200 60 61
21 46709983 2823765494 60 61
22 50818468 2871254034 60 61
X 156040895 2922919533 60 61
Y 57227415 3081561171 60 61
MT 16569 3139742431 60 61
chr1 248956422 56 60 61
chr2 242193529 253105808 60 61
chr3 198295559 499335952 60 61
chr4 190214555 700936493 60 61
chr5 181538259 894321347 60 61
chr6 170805979 1078885300 60 61
chr7 159345973 1252538102 60 61
chr8 145138636 1414539898 60 61
chr9 138394717 1562097568 60 61
chr10 133797422 1702798922 60 61
chr11 135086622 1838826360 60 61
chr12 133275309 1976164484 60 61
chr13 114364328 2111661107 60 61
chr14 107043718 2227931566 60 61
chr15 101991189 2336759404 60 61
chr16 90338345 2440450504 60 61
chr17 83257441 2532294546 60 61
chr18 80373285 2616939669 60 61
chr19 58617616 2698652566 60 61
chr20 64444167 2758247200 60 61
chr21 46709983 2823765494 60 61
chr22 50818468 2871254034 60 61
chrX 156040895 2922919533 60 61
chrY 57227415 3081561171 60 61
chrMT 16569 3139742431 60 61
.....
So my vcf2maf ran perfectly.
Interestingly this is specifically vcf2maf issue, vep alone runs fine at the same file. Moreover, when i tried to convert such vep output to maf with vcf2maf, it gave me the same error until i applied abovementioned hack.
And by the way, it is not only chr10, when i deleted chr10 variants, it started to curse at chr11.
Hi, I am experiencing the same issue as Saya #257
I ran the command:
But I got the same errors:
I have tried with Ensembl's GRCh38 assembly, as well as NCBI RefSeq GRCh38 & GRCh38p13 genome assemblies. Any idea how to resolve this?
Thanks!