Open pblaney opened 1 year ago
Hi Patrick,
Thanks for letting us know about the error. I have not encountered this before on our samples. What happens if you set number.of.samples to the total number of available samples when choosing randomly?
Zi-Ning
Hello Zi-Ning,
I finally had some time to test out your suggestion. Unfortunately, using choose.randomly
with setting number.of.samples
equal to the total number of samples leads to the same error as use.all
.
Furthermore, choose.randomly
works when I set the number of samples to 2 out of 4 but it fails when I use 3 out of 4.
The same occurs with choose.by.clustering
.
I'll keep testing to see if I can determine a pattern or give more information for debugging if others experience the same issue. I plan to greatly increase the input sample size so this may help resolve this as well.
Hello,
After collecting a test set of fragCounter coverage profiles for 4 normal samples, I attempted to run the
dryclean
workflow. I encountered the following error while trying the first step of creating the PoN inprepare_detergent
:While troubleshooting, it seems like others have encountered the same error, but at a different stage of the workflow (#2). Based on the output message, it looks like the error occurs within
pbmclapply
function call at line 259 although I am not exactly sure where.I then decided to test
prepare_detergent
under the other possible approaches instead of using all samples. Interestingly, using either of the two alternative optionschoose.randomly = TRUE
orchoose.by.clustering = TRUE
both executed without an error.Here using
choose.randomly = TRUE
and selecting 2 of the 4 samples:And here using
choose.by.clustering = TRUE
The output
detergent.rds
is in working order as I was able to runstart_wash_cycle
without any problems. I will likely use the clustering method for further analysis but wanted to point out this issue for others who encounter it.Best, Patrick