I have aligned two homologous chromosome with high heterogeneity using nucmer, delta-filter and dnadiff, get the report file with the information:
[Bases]
TotalBases 22071000 22382214
AlignedBases 12953069(58.6882%) 12986449(58.0213%)
UnalignedBases 9117931(41.3118%) 9395765(41.9787%)
(1) How should I understand the aligned bases?
(2) when I calculated the snp percentage, should I calibrate the snp rate by the aligned bases rates?
Dear officer,
I have aligned two homologous chromosome with high heterogeneity using nucmer, delta-filter and dnadiff, get the report file with the information: [Bases] TotalBases 22071000 22382214 AlignedBases 12953069(58.6882%) 12986449(58.0213%) UnalignedBases 9117931(41.3118%) 9395765(41.9787%)
(1) How should I understand the aligned bases? (2) when I calculated the snp percentage, should I calibrate the snp rate by the aligned bases rates?
Thank you very much! Shiqian