Open milkcookie opened 6 years ago
You could try the patch from #30. It makes nucmer faster and use significantly less memory.
!!! 2018-03-12 11:19:17 Doing step 20: Getting overlaps !!! 2018-03-12 11:19:17 Running: nucmer -maxmatch -c 100 292S_more2kb_contigs_nr.ref.seq 292S_more2kb_contigs_nr.qry.seq -p 292S_more2kb_contigs_nr 1: PREPARING DATA 2,3: RUNNING mummer AND CREATING CLUSTERS reading input file "292S_more2kb_contigs_nr.ntref" of length 32244835963 construct suffix tree for sequence of length 32244835963 (maximum reference length is 2305843009213693948) (maximum query length is 18446744073709551615) process 322448359 characters per dot file "construct.c", line 878: allocandusespaceviaptr(8,48367253946) failed:not enough memory ERROR: mummer and/or mgaps returned non-zero !!! 2018-03-12 12:11:09 Command: nucmer -maxmatch -c 100 292S_more2kb_contigs_nr.ref.seq 292S_more2kb_contigs_nr.qry.seq -p 292S_more2kb_contigs_nr exited with status: 1 !!! END - Elapsed time: 0d 0h 51m 52s
Hello, 30 Gb contigs vs 30Gb contigs, how much memory will be used?
Hi thanks @kloetzl , sorry to reply later, I hava install the software Tumer, but I don't know how to use it with Mummer. Hi @alienzj ,I think you can split itfor SV detection, its too big for Mummer.
30 Gb contigs, how much memory will be used?
IIRC, mummer uses roughly 7byte per input nucleotide.
You might have another problem here: You need more than 32bits to represent the length of the genome. I am not sure if mummer correctly handles lengths with 64bit integers (judging from the line with the error, it doesn't).
Hi thanks @kloetzl, sorry to reply later, I hava install the software Tummer, but I don't know how to use it with Mummer.
TUMmer is just a replacement for the MUMmer executable. So you would call it with the same set of parameters. If you have any specific inquiries, please open an issue in the TUMmer repo.
Dear all: I want to identify variation between two similar genome, but when I use Mummer4 it use almost more than 1000Gb RAM, our linux server can't run with it, the larger one genome size is about 525Mb, the other one is about 350Mb, I want to know how can I limit the RAM when I use this software? whether the software have a limit of genome size? nucmer --maxmatch --prefix=test test1.fasta test2.fasta Thanks FAFU milk