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nanoporetech / dorado

Oxford Nanopore's Basecaller
https://nanoporetech.com/
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Modification tags and re-calling #1142

Open wanghlv opened 18 hours ago

wanghlv commented 18 hours ago

Hi, I'm wondering if I'd like to call all modification possible using the rna004_130bps_sup@v5.1.0, will DORADO write out each modification separately in a different field in BAN file or output as different files. Thanks! Also, if I would like to redo-modification call later, do I need to start from POD5 files again? Thanks again!

malton-ont commented 17 hours ago

Hi @wanghlv,

Modification data is stored in the output BAM file as MM and ML tags - see the docs here and the specification here (section 1.7) for more details.

If you want to recall the modifications you will need to re-basecall from pod5, yes.