Hi,
When I was using Dorado to detect 6mA, I output the modification status of each site through Modkit and found that a large number of sites had a high rate of no-call results. According to the Modkit documentation, the undetected results are due to mismatches around the detection sites, such as CG turning into CH, which leads to a motif mismatch. However, 6mA detection should not be limited by motifs, so I am unclear why there is such a high proportion of no-call results.
Hi, When I was using Dorado to detect 6mA, I output the modification status of each site through Modkit and found that a large number of sites had a high rate of no-call results. According to the Modkit documentation, the undetected results are due to mismatches around the detection sites, such as CG turning into CH, which leads to a motif mismatch. However, 6mA detection should not be limited by motifs, so I am unclear why there is such a high proportion of no-call results.
