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nanoporetech / dorado

Oxford Nanopore's Basecaller
https://nanoporetech.com/
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Could Dorado provide the ployA start and end index? #700

Closed guangzhaocs closed 4 months ago

guangzhaocs commented 4 months ago

  1. I add --estimate-poly-a to the basecaller command. In the bam resluts, I get pt:i tag for some reads. But I could NOT find the ployA start and end index according to the raw signal. So in the current version, could Dorado only provide the length of ployA?

  2. When I add --estimate-poly-a --emit-fastq to the basecaller command, in the final fastq file I can NOT find the pt:i tag. So could Dorado only provide the length of ployA in bam/sam format?

Thanks a lot.

tijyojwad commented 4 months ago

So in the current version, could Dorado only provide the length of ployA?

yes that's right - we plan to add boundaries of the signal in a subsequent release.

So could Dorado only provide the length of ployA in bam/sam format?

yes correct

guangzhaocs commented 4 months ago

Thanks very much, I don't have other questions about this.