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nanoporetech / dorado

Oxford Nanopore's Basecaller
https://nanoporetech.com/
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Dorado Demultiplexing #722

Closed akholwad closed 6 months ago

akholwad commented 6 months ago

Issue Report

I am running dorado demultiplexing using custom barcodes, and the majority of my reads end up unclassified.

My custom TOML file includes the following trailing flank:

mask1_rear = "AGRGTTYGATYMTGGCTCAG"

Are variable nucleotide sites (R, Y, M) supported by the dorado demultiplexing?

tijyojwad commented 6 months ago

Hi @akholwad - we don't support variable nucleotides currently, but those can be added in a future release