I would like to confirm whether the reads of the output bam file (e.g. duplex_sup.bam in the below script) are trimmed adapters when running dorado duplex.
PS:
If adapters of reads are not trimmed, which kinds of reads below are not trimmed?
dx:i:1 for duplex reads.
dx:i:0 for simplex reads which don't have duplex offsprings.
dx:i:-1 for simplex reads which have duplex offsprings.
But I think the default of dorado basecaller trimming is enable based on the README as described:
By default, dorado basecaller will attempt to detect any adapter or primer sequences at the beginning and ending of reads, and remove them from the output sequence.
Hi,
I would like to confirm whether the reads of the output bam file (e.g. duplex_sup.bam in the below script) are trimmed adapters when running
dorado duplex
.dorado version: v0.7.0
script:
dorado duplex "/model/dna_r10.4.1_e8.2_400bps_sup@v5.0.0/" ./pod5/ > duplex_sup.bam
PS: If adapters of reads are not trimmed, which kinds of reads below are not trimmed?
dx:i:1
for duplex reads.dx:i:0
for simplex reads which don't have duplex offsprings.dx:i:-1
for simplex reads which have duplex offsprings.But I think the default of dorado basecaller trimming is enable based on the README as described:
Thank you.