Hi,
in version 0.7.2, dorado demux outputs all barcodes in the given kit in fastq format (--emit-fastq) even if the barcode is not listed in the sample sheet. Samples in the sample sheet are named with the alias name (which is perfect) while other will be named something like SQK-NBD114-24_barcode12.fastq, SQK-NBD114-24_barcode13.fastq etc.
Is it possible to limit the output to only the samples included in the sample sheet and suppress others. I think this was like that in the older versions of dorado. In fact the samples listed in the samplesheet are the most important (target) ones while the others have limited values, I mean in routine work
Thanks in advance
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Issue Report
Please describe the issue:
Hi, in version 0.7.2,
dorado demux
outputs all barcodes in the given kit in fastq format (--emit-fastq
) even if the barcode is not listed in the sample sheet. Samples in the sample sheet are named with the alias name (which is perfect) while other will be named something like SQK-NBD114-24_barcode12.fastq, SQK-NBD114-24_barcode13.fastq etc. Is it possible to limit the output to only the samples included in the sample sheet and suppress others. I think this was like that in the older versions of dorado. In fact the samples listed in the samplesheet are the most important (target) ones while the others have limited values, I mean in routine workThanks in advance
Please provide a clear and concise description of the issue you are seeing and the result you expect.
Steps to reproduce the issue:
Please list any steps to reproduce the issue.
Run environment:
dorado-0.5 demux calls.bam --sample-sheet sample_sheet.csv --output-dir <outdir> --kit-name SQK-NBD114-24 --emit-fastq
Logs