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nanoporetech / flappie

Flip-flop basecaller for Oxford Nanopore reads
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running flappie on ubuntu 16.04(run misc/trace_flipflop.py error) #38

Open jacka87164 opened 5 years ago

jacka87164 commented 5 years ago

Hi

I'm getting this error while checking trace viewer.why can i do?

Error message:

Traceback (most recent call last): File "misc/trace_flipflop.py", line 187, in 'BaseCalled_template', 'Trace')][()] File "h5py/_objects.pyx", line 54, in h5py._objects.with_phil.wrapper File "h5py/_objects.pyx", line 55, in h5py._objects.with_phil.wrapper File "/root/anaconda2/envs/py36/lib/python3.6/site-packages/h5py/_hl/group.py", line 262, in getitem oid = h5o.open(self.id, self._e(name), lapl=self._lapl) File "h5py/_objects.pyx", line 54, in h5py._objects.with_phil.wrapper File "h5py/_objects.pyx", line 55, in h5py._objects.with_phil.wrapper File "h5py/h5o.pyx", line 190, in h5py.h5o.open KeyError: "Unable to open object (object 'Trace' doesn't exist)"

Thank

tmassingham-ont commented 5 years ago

Hello. The problem appears to be that the file you are analysing does not contain trace output ('Trace' group). How did you produce the file you are analysing? Both Guppy and Flappie require trace output to be explicitly turned on, since it can slow down base calling and increases the size of files produced.