Describe the bug
Should the sum of reads in SR be equal to DP?
##INFO=<ID=DP,Number=1,Type=Integer,Description="Depth of reads at pos">##INFO=<ID=SR,Number=.,Type=Integer,Description="Depth of spanning reads by strand which best align to each allele (ref fwd, ref rev, alt1 fwd, alt1 rev, etc.)">
Logging
I generated vcf files using medaka variant and medaka_haploid_variant
Describe the bug Should the sum of reads in SR be equal to DP?
##INFO=<ID=DP,Number=1,Type=Integer,Description="Depth of reads at pos">
##INFO=<ID=SR,Number=.,Type=Integer,Description="Depth of spanning reads by strand which best align to each allele (ref fwd, ref rev, alt1 fwd, alt1 rev, etc.)">
Logging I generated vcf files using medaka variant and medaka_haploid_variant
medaka variant genome.fa consensus.hdf output_1.vcf
medaka tools annotate --dpsp output_2.vcf genome.fa sample.bam
medaka_haploid_variant -s -m r1041_e82_400bps_sup_variant_v4.2.0 -r genome.fa -i test.fastq -o vcf_dir
Here's one SNP where the sum of SR is more that DP:
Environment Installation method: Singularity image from https://depot.galaxyproject.org/singularity/medaka:1.11.1--py310h87e71ce_0 medaka version 1.11.1 No GPU