Open franrodalg opened 3 years ago
This is not currently implemented. There is a bit of logic that needs to be filled in to enable variant calling on RNA/3’ to 5’ signal direction reads. This is missing at the moment. This feature is of interest though, so we will look into adding this feature in a future megalodon release.
Thanks, Marcus. It would indeed be really really useful if this could be incorporated into future releases.
Hi @marcus1487 !
I'm aware that the models for modified RNA bases are still in preparation, but is there any way of calling variants from direct RNA data? There doesn't seem to be associated calibration files at the moment:
ERROR: No default sequence variant calibration file found for guppy config: rna_r9.4.1_70bps_hac.cfg
Cheers, Fran