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nanoporetech / megalodon

Megalodon is a research command line tool to extract high accuracy modified base and sequence variant calls from raw nanopore reads by anchoring the information rich basecalling neural network output to a reference genome/transriptome.
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No fast5_out option in Guppy 6.5.7 #349

Open zjzace opened 1 year ago

zjzace commented 1 year ago

Hi everyone,

I currently running Guppy for the basecalling. I found that the option '--fast5_out' was removed from Guppy 6.5.7.

Can anyone provide some information of why this option was not included in this version?

Thanks, Leo

JSluo888 commented 1 month ago

Hi, I am wondering if you have found a work-around for this?

zjzace commented 1 month ago

Hi JSluo888,

I used an older version, 6.1.3