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nanoporetech / ont_fast5_api

Oxford Nanopore Technologies fast5 API software
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ERROR:ont_fast5_api.conversion_tools.multi_to_single_fast5:Could not convert Multi->Single for file type 'single-read' with path #40

Open akk01 opened 4 years ago

akk01 commented 4 years ago

I am getting this error when I run multi_to_single_fast5 with Fast5:

ERROR:ont_fast5_api.conversion_tools.multi_to_single_fast5:Could not convert Multi->Single for file type 'single-read' with path

How to check if the files are multi-fast5? What about the case with mixture of both single- and multi-read fast5.

fbrennen commented 4 years ago

Hi @akk01 -- we don't currently have a good solution for when you end up with a folder of mixed single- and multi-read fast5 files, but we can put one together. You've definitely got both types in your folder?

akk01 commented 4 years ago

I am not sure if the folder contains both single and multi-read fast5.

akk01 commented 4 years ago

I came across a possible solution of the above problem: https://bioinformatics.stackexchange.com/questions/7034/questions-regarding-nanopore-sequencing-analysis

fbrennen commented 4 years ago

A good answer there -- we'll update our scripts to work on both file types anyways.