can this run with single UMI

nanoporetech / pipeline-umi-amplicon

Workflow to prepare high accuracy single molecule consensus sequences from amplicon data using unique molecular identifiers

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can this run with single UMI #33

Open alexyfyf opened 1 week ago

alexyfyf commented 1 week ago

Hi team,

I'm wondering if this pipeline will work if my reads contains only UMI at one end? What read structure is needed to run this successfully? My current read looks like [Nanopore primer] -- [barcode] -- [custom primer] -- [ds-cDNA insert] -- [TSO/UMI] -- [barcode] -- [Nanopore primer]. How should I adjust the config to run this?

Thank you.

Alex