Certain datasets have experimental groups of single samples.
This current breaks differential expression approaches and likely means differential expression will be impossible.
Steps to Reproduce
Create subset of Runsheet rows with at least one group with N = 1 samples
Expected Behavior
Processing workflows should identify these cases and process up to DE but not run DE.
In these cases, normalized data should be available for release.
Actual Behavior
Processing attempts DE and raises exception.
Impact on Data
Non silent bug: no processed data released with this causing an issue.
Known to impact 1 dataset at start of this issue.
Additional Context
Provide any additional information or context that might be relevant to the issue.
Possible Solution (optional)
Process through normalization, then stop.
This will require some modification for post processing which assumes complete processing.
Description
Certain datasets have experimental groups of single samples. This current breaks differential expression approaches and likely means differential expression will be impossible.
Steps to Reproduce
Expected Behavior
Processing workflows should identify these cases and process up to DE but not run DE. In these cases, normalized data should be available for release.
Actual Behavior
Processing attempts DE and raises exception.
Impact on Data
Non silent bug: no processed data released with this causing an issue. Known to impact 1 dataset at start of this issue.
Additional Context
Provide any additional information or context that might be relevant to the issue.
Possible Solution (optional)
Process through normalization, then stop. This will require some modification for post processing which assumes complete processing.