Closed adamturner1 closed 3 years ago
Hi Adam,
Could you also paste the command you typed here?
Best regards, Natsuhiko
Hi Natsuhiko,
I use a wrapper script as follows:
The rasqual command I use is:
Apologies if this is a lot of lines. I used rasqualTools to prepare the input files. The 'exon_start_positions' and 'exon_end_positions' correspond to the start and end of each ATAC-seq peak. If I run RASQUAL on a feature (ATAC-seq peak) individually I don't get this error. Since I have thousands of ATAC-seq peaks I have to use the above commands but unfortunately keep on getting 1.000000, 1.000000, etc. and have not figured it out.
Thank you for your help!
Best regards, Adam
Hi Adam,
If you remove -v
option in the second last line of the above screen shot, what will happen?
Best regards, Natsuhiko
Hi Adam,
I modified the verbose level so that you won't see this line with -v
option. Sorry for the inconvenience.
Best regards, Natsuhiko
Hi Natsuhiko,
Thank you for this!
Best, Adam
Hi Natsuhiko,
RASQUAL has been helpful so far for caQTL analysis and ATAC-seq data. It seems to be working when I use a wrapper script to run RASQUAL on all peaks in our dataset. However, I get 1.000000, 1.000000, etc. all across the first row of each output file (I attached a picture of what I get). The rest of the output all looks correct according to the Output section in the tutorial. I've tried a lot of things but always get this in the output.
Do you think is due to some memory issue or something else? Also I assume this would not affect our results?
Any help would be much appreciated.
Thank you! Adam