naturalis / barcode-constrained-phylogeny

Pipeline for building topologically-constrained phylogenies from DNA barcode data
https://naturalis.github.io/barcode-constrained-phylogeny/
Apache License 2.0
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Picking exemplars #77

Closed rvosa closed 7 months ago

rvosa commented 8 months ago

When picking the shallowest exemplars for the backbone, exemplar clades where shallow tips have near zero distance to their subtree root result in a backbone where nearly all change is allocated to the root branch of the of the exemplar clade. When grafting the subtrees onto the basal split, the subtrees become "too tall". Here's the grafted example:

Screenshot 2024-01-11 at 12 33 27

Here's the same topology but with the branch lengths re-estimated by raxml:

Screenshot 2024-01-11 at 12 34 17

A follow up experiment should be to pick the tallest exemplars instead. It's possible that this will make them "too shallow" instead, especially when there is a lot of homoplasy within the clade.

rvosa commented 8 months ago

Note also that the clade that sticks out very far in the re-estimated tree is rooted incorrectly, which needs to be fixed as per issue #76

rvosa commented 8 months ago

The way to go about this is as follows:

rvosa commented 7 months ago

Picking this up in #79