navinlabcode / copykat

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Diploid and aneuploid reversed #111

Open LeaLe88 opened 8 months ago

LeaLe88 commented 8 months ago

Good afternoon,

I have ran copykat on one tumour sample. I was expecting to see aneuploid in the epithelial/tumour cluster and diploid in the remaining cluster. However, I got the opposite result. Please find the clustering below:

image

image

I do receive the error "low confidence in classification" as described in https://github.com/navinlabcode/copykat/issues/18.

I followed the suggestions in issue 18 and limited my analysis to epithelial cells only. However again I get the error "low confidence in classification" and I see a mix of diploid and aneuploid in the epithelial cells:

image

Do you know what else I could do to obtain a correct annotation of my clusters?

Thank you very much!