I'm analyzing single-cell spatial transcriptomic, more specifically CosMx data base. In this database, I don't have the whole genome but only 960 genes. I would have liked to use copykat but the problem is that most of my cells get filtered out because they express less genes than what is specified in min.gene.per.cell:
[1] "960 genes, 97907 cells in raw data"
[1] "filtered out 82746 cells with less than min.gene.per.cell; remaining 15448 cells"
I think they get filtered out because I have only 960 genes, is there a way to lower the min.gene.per.cell ? I can't find anywhere a way to do it...
Hi,
I'm analyzing single-cell spatial transcriptomic, more specifically CosMx data base. In this database, I don't have the whole genome but only 960 genes. I would have liked to use copykat but the problem is that most of my cells get filtered out because they express less genes than what is specified in min.gene.per.cell:
I think they get filtered out because I have only 960 genes, is there a way to lower the min.gene.per.cell ? I can't find anywhere a way to do it...
Thank you in advance !