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SRA Tools
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i have some problems in using aspera to download sra #53

Closed nickxzshi closed 7 years ago

nickxzshi commented 7 years ago

Hello,dear everyone. I want to download some RNA-seq raw data from NCBI by using Aspera. i used the command

~/.aspera/connect/bin/ascp -i ~/.aspera/connect/etc/asperaweb_id_dsa.openssh -k 0 -T -l200m  anonftp@ftp-private.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/SRR/SRR285/SRR2854733/SRR2854733.sra .
and terminal told me that

ascp: Source file list not specified, exiting

```i check the file in ncbi,the SRR2854733.sra in there . meanwhile, i try the SRR645370.sra

~/.aspera/connect/bin/ascp -i ~/.aspera/connect/etc/asperaweb_id_dsa.openssh -k 1 -T -l200m anonftp@ftp-private.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/SRR/SRR645/SRR645370/SRR645370.sra .SRR645370.sra 
0% 6161KB 9.5Mb/s 3:13:

it is working. can everybody tell me the reason？do the aspera have limit on some sra? I'd appreciate any advice & suggestions.thank you very much.

kwrodarmer commented 7 years ago

Please download or build the latest SRA Toolkit and use prefetch to access the data, or let the tools access the data remotely on demand (depending upon how you are using the data). The hand-crafted URL you are using is deprecated, as is direct use of ascp.

We have a Wiki page on how to access data - https://github.com/ncbi/sra-tools/wiki/HowTo:-Access-SRA-Data . Please see that page, and if you are still having difficulties, write back and we'll help diagnose the problem.

nickxzshi commented 7 years ago

thank you for your enxplanation 👍

Best Regards nick