can't prefetch from AWS instance

[Bu-fetch]

Hello,

I am trying to work with RNA-seq data within an Amazon AWS instance. I've successfully launched an Ubuntu-based AMI and installed various pipeline tools, including Anaconda and SRA toolkit.

However, I am facing an issue when trying to download SRR files using prefetch. Even after referencing the discussion at https://github.com/ncbi/sra-tools/issues/72 and creating a jwt.cart file, I still cannot successfully run prefetch.

Here's the command I used:

prefetch --perm jwt.cart SRR851812

Unfortunately, I received the following error message:

2023-10-05T07:31:06 prefetch.2.8.0 err: param unknown while parsing argument list within application support module - Unknown argument '--perm'
2023-10-05T07:31:06 prefetch.2.8.0 int: param unknown while parsing argument list within application support module - ArgsMakeAndHandle

Any guidance or suggestions on how to resolve this issue would be greatly appreciated.

Thank you!

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[klymenko]

Use the latest toolkit.

[Bu-fetch]

After wget and unzip sratoolkit.3.0.7-ubuntu64.tar.gz, I used $ sudo apt install sra-toolkit then, now I can prefetch SRR files! ex. $ prefetch SRR851812

Thank you!