fastq-dump/fasterq-dump: can't open JTFH01xxxxxx as a RefSeq or as a WGS

sa2adeel commented 1 month ago

I am currently running sratoolkit 3.1.1 and have run into the following error:

fasterq-dump:

fasterq-dump --split-files path/SRR22287940 -e 6 --outdir test

2024-07-13T01:17:07 fasterq-dump.3.1.1 warn: directory not found while opening manager within virtual file system module - can't open JTFH01000001 as a RefSeq or as a $2024-07-13T01:17:07 fasterq-dump.3.1.1 err: sorter.c producer_thread_func: rec.read.len = 0 2024-07-13T01:17:08 fasterq-dump.3.1.1 err: sorter.c run_producer_pool().join_and_release_threads -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull) 2024-07-13T01:17:08 fasterq-dump.3.1.1 err: sorter.c execute_lookup_production() -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull) 2024-07-13T01:17:08 fasterq-dump.3.1.1 err: fasterq-dump.c produce_lookup_files() -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)

fastq-dump:

fastq-dump --gzip --skip-technical --split-files path/SRR22287940

2024-07-13T01:42:02 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000001 as a RefSeq or as a WGS 2024-07-13T01:45:03 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000013 as a RefSeq or as a WGS 2024-07-13T01:48:05 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000015 as a RefSeq or as a WGS 2024-07-13T01:51:06 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000017 as a RefSeq or as a WGS 2024-07-13T01:54:08 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000018 as a RefSeq or as a WGS 2024-07-13T01:57:10 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000022 as a RefSeq or as a WGS etc....

This error occurs in only a subset of the SRA accessions, specially WGS samples. The accessions were downloaded using prefetch and are unfortunately restricted as they are samples from a clinical study. As you can see from the files within the accession folder, the JTFH01 reference files are located within the folder so I am not sure why it is trying to download/create the files again. Also, our compute cluster is restricted from the internet so attempts to fetch any reference files via fastq-dump/fasterq-dump is denied.

JTFH01 KN707668.1 KN707750.1 KN707845.1 KN707912.1 NC_000005.10 NT_167218.1 NT_187387.1 NT_187443.1 NT_187508.1 NT_187545.1 NT_187582.1 NT_187619.1 NT_187656.1 NT_187693.1 NW_003315958.1 KN707607.1 KN707669.1 KN707753.1 KN707850.1 KN707913.1 NC_000006.12 NT_167219.1 NT_187388.1 NT_187444.1 NT_187509.1 NT_187546.1 NT_187583.1 ... NW_004166862.2 KN707661.1 KN707744.1 KN707835.1 KN707904.1 NC_000002.12 NT_167213.1 NT_187384.1 NT_187438.1 NT_187505.1 NT_187542.1 NT_187579.1 NT_187616.1 NT_187653.1 NT_187690.1 NW_003315955.1 NW_004504305.1 KN707662.1 KN707746.1 KN707840.1 KN707905.1 NC_000003.12 NT_167214.1 NT_187385.1 NT_187439.1 NT_187506.1 NT_187543.1 NT_187580.1 NT_187617.1 NT_187654.1 NT_187691.1 NW_003315956.1 SRR22287940.sra KN707664.1 KN707749.1 KN707844.1 KN707906.1 NC_000004.12 NT_167215.1 NT_187386.1 NT_187442.1 NT_187507.1 NT_187544.1 NT_187581.1 NT_187618.1 NT_187655.1 NT_187692.1 NW_003315957.1

I have been stuck on this problem for days now and can't find any resources to help with this issue. So, any help is much appreciated.

Thank you.

klymenko commented 1 month ago

What is the output of the following? align-info path/SRR22287940

sa2adeel commented 1 month ago

Here is a subset of the output (let me know if you want the whole thing):

JTFH01000001,chrUn_JTFH01000001v1_decoy,false,remote JTFH01000002,chrUn_JTFH01000002v1_decoy,false,remote JTFH01000003,chrUn_JTFH01000003v1_decoy,false,remote JTFH01000004,chrUn_JTFH01000004v1_decoy,false,remote JTFH01000007,chrUn_JTFH01000007v1_decoy,false,remote JTFH01000008,chrUn_JTFH01000008v1_decoy,false,remote JTFH01000012,chrUn_JTFH01000012v1_decoy,false,remote ... JTFH01001664,chrUn_JTFH01001664v1_decoy,false,remote JTFH01001669,chrUn_JTFH01001669v1_decoy,false,remote JTFH01001676,chrUn_JTFH01001676v1_decoy,false,remote JTFH01001677,chrUn_JTFH01001677v1_decoy,false,remote JTFH01001996,chrUn_JTFH01001996v1_decoy,false,remote JTFH01001997,chrUn_JTFH01001997v1_decoy,false,remote JTFH01001998,chrUn_JTFH01001998v1_decoy,false,remote KN707607.1,chrUn_KN707607v1_decoy,false,remote:path/SRR22287940/KN707607.1 KN707608.1,chrUn_KN707608v1_decoy,false,remote:path/SRR22287940/KN707608.1 KN707609.1,chrUn_KN707609v1_decoy,false,remote:path/SRR22287940/KN707609.1 KN707610.1,chrUn_KN707610v1_decoy,false,remote:path/SRR22287940/KN707610.1 KN707611.1,chrUn_KN707611v1_decoy,false,remote:path/SRR22287940/KN707611.1 KN707612.1,chrUn_KN707612v1_decoy,false,remote:path/SRR22287940/KN707612.1 KN707613.1,chrUn_KN707613v1_decoy,false,remote:path/SRR22287940/KN707613.1 KN707614.1,chrUn_KN707614v1_decoy,false,remote:path/SRR22287940/KN707614.1 KN707615.1,chrUn_KN707615v1_decoy,false,remote:path/SRR22287940/KN707615.1 KN707616.1,chrUn_KN707616v1_decoy,false,remote:path/SRR22287940/KN707616.1 KN707623.1,chrUn_KN707623v1_decoy,false,remote:path/SRR22287940/KN707623.1 KN707626.1,chrUn_KN707626v1_decoy,false,remote:path/SRR22287940/KN707626.1 KN707628.1,chrUn_KN707628v1_decoy,false,remote:path/SRR22287940/KN707628.1 KN707630.1,chrUn_KN707630v1_decoy,false,remote:path/SRR22287940/KN707630.1 KN707631.1,chrUn_KN707631v1_decoy,false,remote:path/SRR22287940/KN707631.1

klymenko commented 1 month ago

What is the output of the following? ls -l path/SRR22287940/JTFH01

sa2adeel commented 1 month ago

-rw-r----- 1 usr gdc 1322174 Jul 8 16:16 JTFH01

klymenko commented 1 month ago

What is the output of the following? vdb-dump --info path/SRR22287940/JTFH01

sa2adeel commented 1 month ago

acc    : JTFH01
path   : path/SRR22287940/JTFH01
size   : 1,322,174
type   : Database
platf  : SRA_PLATFORM_UNDEFINED
SEQ    : 1,998
SCHEMA : NCBI:WGS:db:contig#1.1

Thank you for taking the time to help!

klymenko commented 1 month ago

Do all other files in SRR22287940 directory have the same permissions as JTFH01? ('Others' cannot read it)

sa2adeel commented 1 month ago

Yes, that is correct.

-rw-r----- 1 usr gdc    1322174 Jul  8 16:16 JTFH01
-rw-r----- 1 usr gdc      17184 Jul  8 16:10 KN707607.1
-rw-r----- 1 usr gdc      17188 Jul  8 16:10 KN707608.1
-rw-r----- 1 usr gdc      16836 Jul  8 16:10 KN707609.1
-rw-r----- 1 usr gdc      16774 Jul  8 16:10 KN707610.1
-rw-r----- 1 usr gdc      16701 Jul  8 16:10 KN707611.1
-rw-r----- 1 usr gdc      16685 Jul  8 16:10 KN707612.1
-rw-r----- 1 usr gdc      16830 Jul  8 16:10 KN707613.1
-rw-r----- 1 usr gdc      17206 Jul  8 16:10 KN707614.1
-rw-r----- 1 usr gdc      16909 Jul  8 16:10 KN707615.1
-rw-r----- 1 usr gdc      17203 Jul  8 16:10 KN707616.1
-rw-r----- 1 usr gdc      17356 Jul  8 16:10 KN707623.1
-rw-r----- 1 usr gdc      22556 Jul  8 16:10 KN707626.1
-rw-r----- 1 usr gdc      17150 Jul  8 16:10 KN707628.1
-rw-r----- 1 usr gdc      17004 Jul  8 16:10 KN707630.1
-rw-r----- 1 usr gdc      16912 Jul  8 16:10 KN707631.1
-rw-r----- 1 usr gdc      16744 Jul  8 16:10 KN707633.1
-rw-r----- 1 usr gdc      22488 Jul  8 16:10 KN707637.1
-rw-r----- 1 usr gdc      16749 Jul  8 16:10 KN707639.1
-rw-r----- 1 usr gdc      16837 Jul  8 16:10 KN707641.1
-rw-r----- 1 usr gdc      17161 Jul  8 16:10 KN707642.1
-rw-r----- 1 usr gdc      17140 Jul  8 16:10 KN707643.1
-rw-r----- 1 usr gdc      16683 Jul  8 16:10 KN707644.1
-rw-r----- 1 usr gdc      16921 Jul  8 16:10 KN707647.1
-rw-r----- 1 usr gdc      16801 Jul  8 16:10 KN707648.1
-rw-r----- 1 usr gdc      16869 Jul  8 16:10 KN707649.1
-rw-r----- 1 usr gdc      16795 Jul  8 16:10 KN707650.1
-rw-r----- 1 usr gdc      16898 Jul  8 16:10 KN707653.1
-rw-r----- 1 usr gdc      17122 Jul  8 16:10 KN707655.1

klymenko commented 1 month ago

What is the full output of the following? vdb-dump -R1 path/SRR22287940/JTFH01

sa2adeel commented 1 month ago

ACCESSION: JTFH01000001
       ACC_CONTIG_LEN: 6
           ACC_PREFIX: JTFH01
          ACC_VERSION: 1
       AMBIGUITY_MASK: 0, 0, 0, 0
                ANNOT:
           BASE_COUNT: 4357270
       BIO_BASE_COUNT: 4357270
             CIRCULAR: false
             CLIP_LEN: 25139
           CLIP_START: 0
       CMP_BASE_COUNT: 4357270
         COLOR_MATRIX: 0, 1, 2, 3, 4, 1, 0, 3, 2, 4, 2, 3, 0, 1, 4, 3, 2, 1, 0, 4, 4, 4, 4, 4, 4
          CONTIG_NAME: decoy00001
CONTIG_NAME_ROW_RANGE:
               CSREAD: 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               CS_KEY: T
            CS_NATIVE: false
                DESCR: 49, 128, 182, 128, 48, 128, 162, 128, 48, 128, 160, 128, 26, 12, 72, 111, 109, 111, 32, 115, 97, 112, 105, 101, 110, 115, 0, 0, 161, 128, 26, 5, 104, 117, 109, 97, 110, 0, 0, 163, 128, 49, 128, 48, 128, 160, 128, 26, 5, 116, 97, 120, 111, 110, 0, 0, 161, 128, 160, 128, 2, 2, 37, 134, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 165, 128, 48, 128, 160, 128, 160, 128, 48, 128, 160, 128, 26, 4, 72, 111, 109, 111, 0, 0, 161, 128, 26, 7, 115, 97, 112, 105, 101, 110, 115, 0, 0, 0, 0, 0, 0, 0, 0, 162, 128, 48, 128, 48, 128, 160, 128, 2, 1, 17, 0, 0, 161, 128, 26, 31, 80, 111, 111, 108, 32, 111, 102, 32, 50, 53, 52, 32, 112, 117, 98, 108, 105, 99, 32, 83, 71, 68, 80, 32, 115, 97, 109, 112, 108, 101, 115, 0, 0, 0, 0, 0, 0, 0, 0, 163, 128, 26, 129, 154, 69, 117, 107, 97, 114, 121, 111, 116, 97, 59, 32, 77, 101, 116, 97, 122, 111, 97, 59, 32, 67, 104, 111, 114, 100, 97, 116, 97, 59, 32, 67, 114, 97, 110, 105, 97, 116, 97, 59, 32, 86, 101, 114, 116, 101, 98, 114, 97, 116, 97, 59, 32, 69, 117, 116, 101, 108, 101, 111, 115, 116, 111, 109, 105, 59, 32, 77, 97, 109, 109, 97, 108, 105, 97, 59, 32, 69, 117, 116, 104, 101, 114, 105, 97, 59, 32, 69, 117, 97, 114, 99, 104, 111, 110, 116, 111, 103, 108, 105, 114, 101, 115, 59, 32, 80, 114, 105, 109, 97, 116, 101, 115, 59, 32, 72, 97, 112, 108, 111, 114, 114, 104, 105, 110, 105, 59, 32, 67, 97, 116, 97, 114, 114, 104, 105, 110, 105, 59, 32, 72, 111, 109, 105, 110, 105, 100, 97, 101, 59, 32, 72, 111, 109, 111, 0, 0, 164, 128, 2, 1, 1, 0, 0, 165, 128, 2, 1, 2, 0, 0, 166, 128, 26, 3, 80, 82, 73, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 163, 128, 48, 128, 48, 128, 160, 128, 2, 1, 7, 0, 0, 161, 128, 26, 22, 112, 111, 111, 108, 101, 100, 32, 109, 97, 108, 101, 32, 97, 110, 100, 32, 102, 101, 109, 97, 108, 101, 0, 0, 0, 0, 48, 128, 160, 128, 2, 1, 10, 0, 0, 161, 128, 26, 19, 66, 108, 111, 111, 100, 32, 97, 110, 100, 32, 99, 101, 108, 108, 32, 108, 105, 110, 101, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 183, 128, 48, 128, 160, 128, 2, 1, 1, 0, 0, 161, 128, 2, 1, 20, 0, 0, 0, 0, 0, 0, 178, 128, 161, 128, 48, 128, 160, 128, 2, 2, 7, 222, 0, 0, 161, 128, 2, 1, 11, 0, 0, 162, 128, 2, 1, 21, 0, 0, 0, 0, 0, 0, 0, 0, 177, 128, 161, 128, 48, 128, 160, 128, 2, 2, 7, 222, 0, 0, 161, 128, 2, 1, 11, 0, 0, 162, 128, 2, 1, 21, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0
         EXTRA_SEQIDS:
         FEAT_ROW_END:
       FEAT_ROW_START:
       FIXED_SPOT_LEN: 0
              GAP_LEN:
          GAP_LINKAGE:
            GAP_PROPS:
            GAP_START:
             GB_STATE: 0
                   GI: 725026265
                 HASH: -604913549
                LABEL: contig
            LABEL_LEN: 6
          LABEL_START: 0
          MAX_SPOT_ID: 1998
          MIN_SPOT_ID: 1
                 NAME: gi|725026265|gb|JTFH01000001.1| Homo sapiens isolate Pool of 254 public SGDP samples decoy00001, whole genome shotgun sequence
       NUC_PROT_DESCR:
             PLATFORM: SRA_PLATFORM_UNDEFINED
           PROT_COUNT:
              QUALITY:
            RD_FILTER: SRA_READ_FILTER_PASS
                 READ: CCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCTCCTCGGCCTCCCAAAGTGCTGGGATTACAGGGACAAACCACTGCGCCCGGCCAGAGCATATTTTAAAAGCTACAATAATTAAAACAATGTGGTATTGGAAGAAAAATAGACAAAAGTATAAAAAAAGTCAAGAAACAGCAGCAAATATACACAAGAATTTACTATCTGATAAAGATGACATTTTAAATAAGTGAAGAAAAGATCAATGTTTTTAAGCAGTGATGTCAGCCCAACTGGAAGAAAATGAGCTGGATTTCTAGTTTACACTGTATACCCAAACAAATTTTAGAGGAAACAATTATACATGTGAACAATGAAATAATCTTAGATGTCATACCTAAGAAATCTTTGCCTAGCCTTACAGAAAACACTAGAAGAAAAAAGGGTAGAATTTTTTTCATAATTGGAGTGAGGAAAAAATCATAAAACCTAGCATCCATAGAACAAAAGATTGCTACTTCATATATATATTTCTCATTTTTTGAACAGCTTTATTGAGGTGTATTTGCTATACAGTAACCCTTACATATATAAAGCAAATGATTTGATAGTTTTTACATATGTATACAGTTGTAAAACCACCATTGCAATCAAGATAACAAACACATCTGCCACTCTCCATGGCTCTTGCCCTTTGTAACCTGTCCCTCCAACCCCGCCCCACCCTGCTCCCCCATCCCAGGTGCACTCTGGTCTGCTTTCTCTCACTACTGACTAGTTTGCTTTCTCTAGAATGTTCTGTAAACAGAATCACACAGGGTATACTCTATCTTGTCGTATTTCTTTCACTCAGTAGAACTATTTGGAGATGTATCCATGTTGTTGCGAGTATCATTAGTATTTTCCTTTTTATTCCTGAGTAGGATTCTACTGTACAGATATACCTCCATTTGTTTATCCAGTTATCTGTTGATGGACATTTGGGTGCTTTGCAATTCGGGCTGCTGTGAACATTAAGCATACGTCACTGTATGGACGTGTGTCTTCATTCCTCTTGGTCTGTCTGAGCTCCACACCTAGGGAGGCTTTGGAATTGCTGGGCCATGTGATAAGTTCTATTCAACGTGTTAGAGAAATTGCTGAGGTGTTTCGCAAGTGGCTGCCCCATTTTCCGTCTCCACCACCAGCTTTTGATGGTTCTAATTTCTTCACGTCCTCACCAACACTGTTACTGTCTGTTGTATGTTTATTATTATCATTGTGGCCATGCCGGTGGGTGCAGTGGTATCTTGCTGTGGTTTTTATCTGCATCTCCCTAGTGACTAACGATGCCGAGCATCCTTTGAAGGCCACGTTACCATGATGACATCTTCTTTGGTGAACTGTCTACTGAAATCTCTTGCCCATTTTTTGTTGGCTCATTTGTCTTTTTATCATTCAGTTTAAGAGTTTTTAACATACTCTAGATGCAAGTCTTTTTTATCAGATATACGATTTGCAAATATTTTCTCCCAGGCTGTGGCTTGTCTTTATATTGTCTGAACAGTATCTTTTGAAGGCCAGAAGTTCTTAATTTTGATGACATTCAATTTATCATTTTTTCCTTTTGGTGTCATGTCTAAGAAATCTTTGCCTAACAACATAAAAACTTGAAATTTTTGAATTATGAAAAGGAATAGAGACAAAGAGAAAAGATAAATGAATAGATCAAAATATTTGAATTCATAGTATAAAGTGCTAATTTGTCTAATAGATAAACAGCTTTCACAAATCAATAAGCAAAAGGCCAGCCATCTGACAGAATGCATTGCCAAGGAATACAAGCAGACAGTTCACAGAAAAGGAAATACAAATGTCTCCTCCACAGAAAACTCAACTTTACTCATAAAAAGAGAAACTCAAAACTACAGCAAGATTTCATTGACTTAACCCTCAAGTGGGCAGAGATCAAAAAGTTGGGTGGCCACTGGGTTGGTAAGGAGGTAGGGGGTAGGAGATGGACTTTCTTACAGTGTCTGACAGAGCATGTATTAGAACAGAATCTACAGAGAGCCATTTGTCAATTACCTGTCAATACAACATTCCTTTTCATCCAAAATTGCACTTCAGGAAATTTATCTTTTGCATATACTGACTCATGTGCAAGATGTACGTGTTCAAAGATACCCATTGCAGCACTGGGTAAATGTGCAATATTGAAAGAAATCTATCAGCAGGAGACTGATCAGGTAGCGTATGGCTGATTCATACAATCAGACAATGGGACGTTGCTGGGGGAGTGTCCTCCCCTACCCAGGTTCAGGTTTCAATGTGCAGTAAGCCCCCTGCCTGGCTTTCTGTCCCAACCCCCAGTTAAAAAAGAGAAAGGGGCTCAGCTTCACAAATGGACGAATTAGAAGACACATTTCCAGGAGGTTCCCTCCTGTACCTTTTGAGTTTTATGCATATACTGTCATTCAAAACAAAGCAAAACTGGCTGGGTGCGGTGGTTCATGCCTAAAATCCCAGCAGTTTGGGAGGCCGAGGTGGGAGGATTGCCCAGGAGTTTGAGGTATCAGTGAGCTATGATTGTGCCACGGCATTCCAGCTTGGGTGACAGAGCAAGACCCTGTCTTAAAAAAAAAAAAAAAAGAGAGAGAGGGAAGAAATAAAATGGAAATCAGCATTTAGGCAGCGCTTTCAGACTGTGAGCACGCTGAAAGAAGAATGCACAATCATTAAAGAAAATGCACAATTATTCATCACCTACTTTGCGCTAGGCACTTTCTTCTAAGCCTCTCAACAACCCTGCAATAAGTGCTTATTATTCTTTACCATTGGGAGCCCAGGTTCAGAGCGGCAGAGCCCTTGCTTGAGGTGGCTCAGTTCTGTCTCCACTGACACCTGCCTAGGAAGCTTTACTTCCAACCTCCTGAACACACAAAAACACACTTTATTAGTACTTGTTTCCTTAATGCCTGCTGCAGGGTCTGGCCCTAATAATCATGCAGTAAGTTCTTGTAGGGTCACACAGAGGAGGTCAATGTTGGTGAGTCTCTCATCCCATCACATCGAGGCCGTTGTCCCCTGCTGCGCCCCAGCCAAAGGCTCTGCACATTACTCTGGGCAGAGATTGGAGATGCTGCCTGGGCCCTGATTTTGGACACCCAGCCCGGCACCATGTCGGGGCCATCCTACTCCAAAGTCCACCCCCTTCCTTCCCTGGGCAGAACCAGGAGCTCCATCTCTGTGGCCTGGGCTGAGGTGGGGGTCACCAGGCCCCTCACCCAGGCTTCCCTCTCAAGCACCAACATCCTAAGCCAAAGAAAACTATCCAAGGCTCTATTGTGGGGCTTCCTACAGCTCTTAGGGGAAGTGCAGATGAGGCACTGGGCCACATAAATCTTCTCCAGTGTCCTATAAAGCAGCTCACTTGGCCTCCACAGCTAGAACATTCCATCCCTGTCTCCCAGGCAGCCTCCCTTCCCCAGGATGCCCGGACACCCCTGCAGACACAGGCGGGCACACTCGCTCCCTCATGGCACTGTTACCACTGGTAGATAAGCTCTTCACGAGGAAAGGGACGCACCTGCCTTGTTACTACCGTGTGCAGGGTACCCGGCACCGTGTCTACAGTGAAGTGATGTAGGAGCCGGCTGAGGGATCACGAGTTTCCATTGCTGGAGGTTGAGGGCCCCCACCTGCCAGTCACACCAAGGCTGGTGAAGCTGCCAGCACCTGCCCAGCAAGGGCGGTGAGGCGCAGATCACTGACGGCCTATTTCGGAGCTGTTGGGTATTTGGTAGAACATTCCAAAAAGCAGAAGCTACCAGCAAATGTAGTCTAAGCCTTGAAACTTACTTTCGTTTTTGGATAAGATGGAGTCACCTTATCATGCAGGTTGCTACCTAAGAAAGACGCCCCTTCAGTAATCCCCACTTCTGTAAAACACACCTGAGGTTTCACCACAAAGCTCCCAAACACATTCGTTCCAAATTGCTTAACTACAGGATCTGCCAGAATCAGCCTGAAGCCCCGCAGTGGCGAAGGAAACGCCGACCGAGTCACCAGGAGGAGCACGTGCGGCCCTAGCAGGCGCGTCCGCTCCCGACTGCTGTGCACGCCGCGTGGGGCACGGGCTTTGGCGTCAGCGTTGCTCGTTTTGACGGGAATCGTTATCATGAAATCTTGTGACACCCACGCCTTGTGCCCTGGTTCATGCAGAGATTAAGAAAAAGCTCCTTACTGTTCATTATTTAAATTAGCCGGTCGGCAACGGCTGGAAGACACCGTGCCCGCCAGTGAAGAACAATGCCAGGCCGGCCGCGCTCACGCCTTCCCACTAGAGGCGGGCCTGGGGGCAGGGCGGGCCTGGGGGGCTGCTCACAGCCCTGGGGGCCCCTCTCGGTGGAGGACCCATTTCCTGTTGTGGAGATTCTTCCCTTTGCAGCCAGGAGAGGGGTCCGGGAGGGCAGATGTGTGCACAGCTCCAACTTCTTGTTTCTCCTTTTCCACGGAGAACATCGACGGAGCGGAAATGTTTGCCGCACGCAGAAGCCTGCAGCTGCATCTCCTCTCCGCCCGCTCCTCGCCCATCAAGCCTTATCGATCTGCAACTGCATTTCGCGCTGAGGTGAAAGTGTTGCGCGCTGATGTGGAATTCTAATCTTCACCGCCCGCTCCCCGCCTGGCCACGGGCGCCCCTCGCACCCCTTCCTCCCCACCCAGCCGGTCCGGCGTGCCTCATTGCAAGTAATTATGCTGCACATGAGTCATTTTTTAATGTTTGGTTTGATACATACAGATTCTTTAAACAATAAAAAAAAATCAGCAAACAGCATCACAATCTGGAAATCTCTTTCTTAACATGGAAAACGGTTTCTTAATGGCACAGGGGGGAACTCAACAGCAAGCTTGACAACATAATGAGGAGATTGTTCCTGGGAAGGGAAGCGCGTTCTTCATGCAGCTCTTACTACTCTCAATAAGATTATTCTCCCTGAATAGCTAATCAGCTTTCTCCAGCTCCCCAAATCCGCGTGCTGGAGGCTGCTCACACGGCTCTCCTTACGCACTAAGTGGCACACAGTGACACCTCGGAGGGCGAAGTCTAAAGTGTTAACAGTATGTGAGCAAACAGCGCAATGGAGGTTGCAGAGAGAGAGGCCTTTGGCAGATGAGCAGCAATTTAGGAAGACGGCTCTGTCACACCTGGGTTGACAACGCTGACAGGGTTTGTTGATCTCTGCTCCCATCTCGAATTTGAGTGTCACGTTTCAACAGAAACGAGAGAAATTAAAAAAAAAAAAAAAGCTTTCAAGAGTCAGCGTTGTTTGCACGGTGTTTGTGACACCGAAACACAAGAGCACGGGGTGGGAGCTGCACTGACCCCGCCTGCAGCTACCCCCACCCTGCCAGAGCCCGCAGAACTGGGTGCAGGGTGAGGCCCGTGGGTCCGGAACAGGGCAAGTGCCCACGGCTGCTCGGGCCCCTCCGGAGCATGTGGTCCTGGACAGTGACACACTTCAAGCCCTGAGCTCACTTGGGAATAAAAGGGCTGATCACAGCCTTGCCTCTGGTCCCGTCGTGGCCCCCACGTTGTTGTCTCAGGGCTTCCGTAGAGGTTGCTCTTCAGGACAGATGACAGGGACCCCAGAGGAAGGCCTGGCCAAGCGTGGGTGTCACCAGACTGCAGGGAGTGAGAAGAGCTGCTGGAGACTGCCCGGCAAGCTTGCCTGGTGCCACAGCCCAGCACATGCCCCACTTTCAAGCAGGTGGCGTCAGACTGTCTGTAGGGCGTAAGAGCGTCCTGGCTCTGCAGGGGTGGCTGAAGCCATTGCGGGCATGTGGAGCCTCATGGAGACGGTAGCCATTTGGGTCCTGAAGCAGCTTCCAGCCTGTCTGGAGCTCTCAGAGTGCCACAAGCACAGATGCAGGGCGTTCTTGACAGCTCACTGAAGACGTGCGTGTGCATCACCCCACGGGTCCCTGGGGCTCCGGGCGTCCCATCTCCATGTCCAGGGCCAGAGGGAGAGGAGGCAGAGCCAAGCCCAGGTGTACTCTAGTGACCCACAGCCCTGAAACACCATAGCCTCATCCAAAGAAAAGCAAGGACACTTGGTGCCTGCAGAACTGAACTGCGCTGGATCCCGCCTCAGAGGCTGGAGAAGAAGGCACACCGGTGGGGCCAGCATGGGGGCCAGATAGCCCTGTCCTTGCCCCTCACTCCGCCTGACTGAGCGCCTCTCAGTCCAGGCAGCAGGAAGCCTCTTCTCCTTGGGAAGCCACTGGCAGTCAGGCTGGCTGTGTGGGGACTTGGCTTCATGCTCCAATATTTGGAAGCTTGATAGAGAGCTTTCACCCTCTACTTGCTTTCTTCCACGTGAGCCCCGTTTTCCCCGGGTGAGAATGCGCTTTGCCGTATTCAGGGTCTGAGCAGCATGTGCCCATATCCTCTCTCCCAACATCCCCATGTCGGGCCCAGAGAGTGCCAGGGCCTGTGGGCTGGGAAGCGGTCACGCCCAGGCGCATTCTGCCCATGCGGCTAGTGCCGTCTGAGAGAATGCACAAGGCGGCCTGCGCTGTGGGCCCTCTGGTCCCAGAGCCACAGTGTGAGTCATGTGCTGCCTCATTGTGAAATGCCCCTGCCCTCTGCCCGATGCAAGGCCCTGGGTGCACGCATGACTCATGGTGGAGGCAGGAGGAAATATGCAAGTTTCTCCACGAACCGGGTGCAGGTGGTCGTGACCTTAATGCCGTTTCCACAGGGGGCCACAGAGACATTCTTTGCAGATTTTTGTGAACACCGTACTCTTGGAGGCCTCCACTCTCAAGCTGGTCCCCAGATGGGCCCGGTAGAAACTCACAGCAAACATGTCTTCCCCAAAGGAAGAATGACAACAGCTGTCTTATTTTGGTGAGGGTAGTTTACAGGGTAGTTTTGGTTATGTTGCAGTGACCCCAAGGTCAGAACCTCAGAGCCGAGATAACCGTTGGAGCTGTGCTCCCCACGCTGGCTCAGCCCCTGGAAGCCTTGGTCTCATGGCTGCCCCGTGTCAGCCGATGGTTCCCAGATCATCAAGACGGTAAGCAAAGCGATGTGGGCACTCCAACCCGCCAGGTCCTCAACATGTCCATGCAGGAGGTACACATGTCCACATCCCACAGTCACCAGCCCAGCCTGGCTTAGCTGAGAAATGCAGCTCTTGTCTGCATGCAGGAGACAAGGCCCAGCAATGAGTCTGAGCAGCACCAAGGTCCGTCCTGTGCGTCAGCTCCCAGGTCTCGGCTCACTGTGCAGGAACCGGAGCTTGATATTTACGACATCTCCTAGGTCATGCACTGTTCATTCATCCGAGGACTAGTTATCGGGAGCTTGTGTTCCTGGAGCTGTGCTCTCCCCTTGCCAGCCTCAGCTCCTCTTCCACCCCAACCTGTGCCCACCACCCCCACACCAGAGGGAAGAAACAACCCCGACCTCAGGTATAGAGTGAAGTCCGGCTGATGCTGGGCTGAAAGAGTAGCTGGATTCTTATTAGGACACAGTAAATGGACCGCACTCAGGAACACGCTCACATCCCAGTCTCTGGATATGGAGGACTTGTGTGTACTGATTCCCCCAGAAACTCATGGACCATGAAAATGACTAAGTATCTCATCTGGCAGGATTTTCATTTGCTCATTGGTATTAGGACAAACAAATGGAAACATCTCCTCTTATTATTCACAGCAAATATGTGGCCACTGCTTTCCTCCCTAACTTCTCAGGCTTAACTGTAAATGCGAATCTCCTGGTGTTTCCTGGAGCTTTCCCCTTCCTTTGTACCATAGGCCCCACATGTCACATTCCTCTGGCACCTCTTTTCCTGCCTTACAGTTACTATCGGCCCTTGTCATCCCCACATCAGGACGCTGAGCTGCGCATGCTGTGTCTGGGCCACGGTCCCCATGAAAATCCTTCCCCTGGTGTTCTGGGTGCCGCCCTCCGGCTGTGCCCTTCCATCCTGGGAAGGTCTGACTGGGGTCCCACGTCACCACGTGGTGGGCCCCACATGCCAGTTCACTCTCGGCTTCCCAGCGCCTCTCTTCCTGCTGCCCTCACCTGCTCTTCTGAGGCTCCTGCAAGGCCCAAGATCCGTCTTTGATGACATTTTTCCTGCTCTCGTTCCTGGCTACAGACTGTTGCCATTTTCCAGCAATGGTTTGAAATAGCATTAGAAAGTTTCATCGAGAACAGAATTCTCTGCCCTACTTCAACATTACGTGTAATCCTATTCTCTGTGAAGCCTCCCCAGCATCACCAGTGGCCATGGAAGTCCCCATCCATGAAACTCCCATGTGGAAACTCCGTAGAGAGACCTCCCATCCCGCTGTGAAGTTCCTGCGTGTCAATGAGAGGCCGGGTTGGGGTAAGATGATCTGCATTGATTTTGAGTCACTGGATGGGAAGACACCAGGGCTGAGATCATTAAGGAAGCTTATTGCTCCGGAAATACACCTGGGCAGAGGCATGTTGTTGACCTCAGAAGCCACGCTGCCTTCCCACACCCTGGTTATTTAATTTTCTTTTGTACTAACTAAAATCAGATTTCTCATTGAGGGTAGAATTTACTGACTTTTCGGATAATACGGAAAGCACACGGGAGGGCTCACCTCATCCTCTGCAATGAGGTGTGCGCAGCATCAGGGTCTGTCTAACCAGGGTCCCCACGGCCAAGCCAAATGAGAGGAGTGCAGACTTCAACTCACAGCAAACCAGCTGAACCCAAGCCTCAAGAGCTTAAAGATTTTGTTAACCAAGATCCACAGACTGAGGACAAGGCAGTTATGCGCTTGAGCTGGCAATAGCCACTGAGCTCAGCGCTCAGCCTGGATCACAGACGGCAGCACCACCATGCTCGGATGCACAGGGTGCGGGGCAGGAGCGTTCAGCCTGGATCACAGACGGCAGCACCACCATGCTCGGATGCACAGGGTGCCGGGCAGGAGCGCTCAGCCTGGATCATAGACGGCAGCACCACCATGCTCGGATGCACAGGGTGCCGGGCAGGAGCGTTCAGCCTGGATCACAGACGGCAGCACCACCATGCTCGGATGCACGGGGTGCCGGGCAGGAGCGCTCAGCCTGGATCATAGACGGCAGCACCACCATGCTTGGATGCACAGGGTGCCAGGCAGGAGCGTTCAGCCTGGATCACAGATGGCAGCACCACCATGCTTGGATGCCACAGGGTGCCGGGCAGGAGCGTTCAGCCTGGATCACAGACGGCAGCACCACCATGCTCGGACGTACGGGGTGCCAGGCAGGTTTGATGTTTCTGTTGATTTTGGCTTCAGCAGTTGAACTCTGGCCTCCATAGGAGGCCCTCCGAGGCCGGAGGGGCCCACACCCTGTATTCCTGCACTTAGTGTCATCCCCGGGCTGGTTAGAGGCTGTCGAAGGTTGGTGAAGCCCTTTGAGCCATCAGGCTGAATCAAGCCTGCAGTGACTTTGTCATCTGTGAAGGATCCTTTCACGGTGAACAAAACTTCAGCTCATCCCCAAACAGACAGACAACATGCTCCTGAGCAGATACGGCATCCTCTGTGGCTCTCCACGCACATCGGAAACATTTTCCTTCAAGCGAGATGAAAATGTGGCAGCTACGCCAACAGCCAGTGCTGAATTCTGTCTCCAGCGAAACGAGGATGGGTGGCTGAAATGGACAGATAATCTAAAAAGGCAAACTTTAGAACACAGAGTATCAATGTGTTCCAAAGCAAACACTTCTTAAGTACCTGGATTACAGGACTTGAATTTCATTTTCTTGTCTTTAGTGCAGAGGAAGCAGACAAGAGAAATGTCTGTGTTAACCTCAAAGATGAGAATTTGGGGTCAGTTCTCAGGGGACAGCCTGTGGCCTGTGTAAACCTCGGCAGTTTCCCTAATTTCTCCCAGCCTCTGTTACCCACACGGCCACCCTGGAAGGAAGACGGGAGGGGTTGTTGGATAGAGAGTTCGCAGTGCGTTAATGAAGCACTCTTGGAGCACACGGGAAGGAGGGATTAGCTCTCACATTGAAGGTGGCATGAAGGCAGGTGCCAGCATGAGGAGGAACGGGAACCCCAGGTAATCCTCCAGTGGCGTCACCTGCCCTCTGGATAACTGAGAGGTGACTAATCTTCTGTAAGTTAATTGTCAGGTGTGGAAAGATTTTGCCCACGGTGAGAGTAGCTCCCGTACGGCACAAAAGGACGGCTTGATTTCCTGCACTTAACACGAGTCACTAGGAGAATCATCTCATCCACCAGCTTCTCCAGTTTTATCAATTATTTATTATTTCCCCCATGCCAAGTTACTTCCATGCATTCCACCCTGTGACAATCACGGAACGCCTGGCTCCTTGTGGCCTGGCAGCACATGGGGCGGCTAGCCCGGACCCTGTGCACACCACCTGGCACGGGACCTGGGGTGGGTGTCTCCCCAGAACCCAGCCCTGACCGGTGGCAGCATGAGGGCCGGTGACCTCTGCGAGAAGCTCGGGGGGGCCTTGCGGTCAGGTCATGTGAGCATGGTTAGCCAAGTGGAGAAGAGCAAACTTCTGGATTCCTAATCAATTCACCTGTTTTGAAATAGAGAGGGATGGTCCCACGGTGAGCACACGGGGTGGGCGTGGCCCTGCTCAGTGCAGGTGGCCAGCCTTGGACTCACACTGGGTGGCCCTCCTGTTCTCCGGGTTCCGCTTCCCAGGCTGTTTCTGTCATTCTGCACCAAAGACCTTCATTCCAGGAGTTACCTACTTAGGCTAAATTGTGAGAATAAAGTGGAGATGGTAGAATTTCCCAGGAGCCGTGCGTATCTTGGGGAAGCGTTTCTTCTCCCAGCCTGTGTCCAGCGTGGGTGGGCTGCGTGGCTTTGTGGATGGAAAAAAGACCCTTCCCATGAAACGGGAGCCCCTCCCCTTCCAGGTTGCTGCAGTCTGTCCTCAGAGAGCCATGCCTTTGACTCACTTTAAATGAGATTTTCTCTTCCCCTGAGGAGAATCATTAACAGATTAATCTAAAAGATTTTCAGAATCTTGCTTGAAAGGCAGATTTTCTTAGAAGAAGAAAAGGAAAAAATAGAAAATTCTGGGTTCCCAAGGTTGGGGGTTGGGAGGAGGGGGCATGGCATCCCCAAAAAGTCAAAGAGTAGAAAAAGAGATGCCGGTGCACCTCCAGGGAAACGCACCTTAACTTGGATGCTCCTTGGTATGTATCCATGACGAGAACAGTGAAGGGCTTGACAATGGGCCAGTCCTACATGCCTGCAGGCCACACCCCCATCCACTGTCAGCAGATCCCCTCTGAGGTCTCCTGACGCCACTCAGAGCAGGCCAGACTCTGTCAGTTCTTCTCTCAGTTCCTGGCATCCTGCACCTGAGGCTTTGAGCACAGCTCAAGTCCCCTCTCCCATTCAGGTTTCCTGCTTGCTCCAACCAGACCCGTTTGCCGAATCCCCTGGGCCTCGCATCCGTGTTTCTATCTTGGCAGTCACCTTCTCTGTCTGGCTGCCCCTCCCCTCTTCCCCTGTGCAGTGGGAACTCTAGATGCCTGTTGAATTGCATTATCATATTCAATGATGAAATTGATGGACAGCAACAACACTCAATGATCAGCAGTAAAATGAAGAGTTACTAACTTTATGGTTAGGTACTTGGCCAATACAATTAAATAATAATTTCCTCCTTTGTATGTGCACCACAATTAAAGGATAAACTCTGCATCAGCAAATGGAGTGTTTTCAGCCAAAGTCAGTCTTAGTCCTGTGACACCGTGGAGCTGGGGCAGACCACCCCCACCTTCCTGGGAGGCTGCACAGGGAAATAGAGAAAACACGGAGATTGGCATAGAGATTCCTTCTCATCCTCAGGTTCCTGACATGCAGGCTGGATAGGACACCACCTTTCCTGGCCATCCTGGAGGGCGGAAAGCGGTAACTGGAGTAGAGTGCTCAGTGACTCATGGGCATAGGTACCCATCACACGTGCAGTGGAGATTTCATTCCCCGGGCTGTCCTATCAAAGCACAGACTGGGTAGCTTAGTACAGAAATTTATTGTCTTGCAGTTCTGGAGGCTGGAAGTCCAAAATTAAGGTGTCGACAGGGCACTGCCCTCTCTGAACCCCCTGAGGAGAATTTTGTCTTGCCTCTTCCAGCTTCCCGTGTGGCCGGCGAGCCTTGGTGTTGCTCAGCTGGTAGACGGTGTCTTCTCCCTGTGTCTTCGCATTGTCTTCTTTGCGTTCCTGTGTCCAGACTTCCCTCTTTCTAAGACACCAGTCACATGGAATTAGTGCTTGCCATAGTGACCACCTCTCAACTTGATGATCTCTGTAAAGTCTCTATTTCAAATAAGGCCACAGGCACAGGTTCTGTGGGTTAGGACTTCAACATAACTTTTTGGGAGGGGATGCAGTTCAACAAATAACAATTGGCTCCCTGACCAACCTTAATGTATTCCTCTCCCCACCTACCCCAAACACTGTGCACTGTGAGGCCAGGTGAGGTCAGTGGTTCTCAAACTTTTTTGTCTGAAGAACCTTTTACACTATTAACAATCACTGAGAAACAAAATGCTTGCTTTTCCCCTGCAGGATATATCTATTGATATTTACCAGGGTAGAAATTAAAAATAGGAAAAATTTTAAATATTCATTTTTAATTAATTAAAAAAATAATAAACCCATTACAGATGCCCCTTGACTTTTCATGGGGTTATGTCCTAATAAAACTATTGTGAATTGAAATTATAAGCTGAAAATGCATTTAATACACCTAGACTACTGAACATCACGGCTTAGCTCAGCCTACCTTAAAGGTGCTCAGAACACCCATGTTAGCCTCCAGTTGGGCAAAATCATCTCACACAAAGTCTATTTTATAATAAAGTGTTGAATAGTTCAAATAATTTGTCGAATACTGAGCTGCAAGTAAAAAAGCAGAATGGTCCTATGGGTGCACCTGCTATGGTCTCTACTGAGCGCGTGTCACTTGAAACCATCCTAAAGTTGAAAAATCTTAAGTGGAACTACTGTGAAGTTGGTGAACATCTGTGTGGAAGTAACTGTATTGTTTGAAAAGGAGTGAGAAGAGCAGCATGGCATCACATTTTTAGAAATCTCTAATATCTGGCTTAATAGAAGTTGGCTGAATTATGATAATGGCTTCTTTCAATCTCTTGCAAAACATTGTTTTGGGGGAAGTATAGGAAGAAAACCAGCTTCTGGCAGATGTGTGTGGAAAGGGAATGCGTATTTTTATAGGCTTTTCCAATCATTATGGATATTCCTTGTGGCTACAAAAAAACTTGAAAAATGGTAGTGCTATAAGATTAGTTGTGGAATCTAAAGCCACACCAGTTCCCTTTTTCACTCTGTTACATCACAAGTCATCAGTCTGTTTGCATGCATGATTTTGTACTTGGACACTATCATTAAAAAATATTGTTTCAGTAAATTTTATACACACACACACTCACACATATATGTAGAGACAGAGAGTGCGTGCACGAGAGAGAGATGGGATTTTTCTCTGTCGCTGAGGCTAGGGTACACTGGTACAATTGTGTGATCATAGCTCACTGCAGCCTTGAACTCCTGGGCTCAAGTGATCCTACTGCCTCTGGCTCCCAAAGCACTGGGATTACGGGTGTAAGCCACCGCATCCAGCCTAGACAATATTTTGAAAATTACATTTGTTAATACCACACATCTCATCAGAAAAGTCTTTAGATCGAGAACATTTCAAGCTCATCGTGGCGGGTATGTTTTCCAAAACTCTAATTTTCACTTGAAGGCTGAAATTTATTATTGGCAACAAATACTGTCAGTTATTTTCTTTGAAGTGATGTTGTGGAGAAAATAAGTAGTGTGCGTTCGTTAAGCATTCTTTCAGACAAAGATGGGATTTCACGGAGAGTGGACAGTGCAGGATCCAGCTGGAACAATCACACGTGCCTCCATCCCTCAGTTTGCAACAGCTTGCACTCCCCATCTTCACATGGGATGTGGGAGAGACAAATACACAAGGGCCAAGGTCTAATACGTTAAAAGCTTTCAGTGCTTCATCCAGAACACCCTATGTGAAAGTGGCTTTTTTCCTGCAAGCGCGTGGTAGGGACCCATGTCGTGGCTCCTGGTGTGGTTGGGCGCAACCACGTTGGCGGATGCTGAGCTGCCATCAGTGTGAACGTCAGTTCGATGAGAGGGTGCCTGTTGAAGTAACATTGCTGCACAGCAAATTACCACAAACGTAGCGGCTACAAACAACCCACAGGTGCCATCTCAGAGCTTCTGTGTGGGTGGCCGAGGCTTAGCCACACCCTCGGCAGGTCTCCCCCGGCTGCAATCGAAATGTCATTTGGGGCTGTAATTCTCATCAGGTCTCAGGTGCGCTTCCAAGATCACATGGACAGTTGGCAGACCTCCGCTTATTGGGGTGATGGCTGAGGACCCTGTCCTCTTGAGAGCTGTGGGCTGGGGGCCGCTCCCAGCTCTGAGATGCCCCTCCCTGTAGATGGCTCACACCATGGCTATTTACCTTTTCCTTTCAAGAGAATCCAAAGAAAAGCTCTGCTTTCAAAGGGCTCACCCGATGAGGCTAGGCCCATACCAAATCATCTCTCTCGATTAATTTAAAGCCAACTGATCTGGGGCCTTGATTACATTTGCAATGTTTTGCCACTTAACATGATCACAGGAGTGATAGCTCATCATTCCAACCCTGACTCAAAGGGAAGGGACTCTACAGGGAGCCTACAGCAGGGTGAGGGTGGAGGAATCACAGGGCTCTCCTAGAACCCTCCCAAGTTTTAAGTCAATACTACCATCGGGACTGGCTACGTAATTTCCAGGGCTCAGTGCAAAATGAAAATGTGGGGGCTCTTCTTCAAAAATTATTAAGAATTTGAAGACTAACAGCTGAGCATTAAAACAAGCATAGCACCTTCTATGCCAGGATCCTGGATGTCTGCCCAGGTCACAGGCTCATGAACATGGCCCTGACTTATTCGCCAACAGTTTTGGTCTTGCAGACACCTCAAAAGGTCCCAAAGACCCTGGGGGATTTGTGGGCCATCTTGGAGAACTGCTGAGTCAAAGCAGCTCAATAATTGTCTGTGGGGACATTTGTCTGACCTTAGTCATGTAGGAAGTCTAAGAAGTTTCTAGCAGCAGTGAGACATCACAGGCCCTTCTCTTTTCAGAAGTCCCTTCTTATTTTCCCTATAGCACAGGACAATTTCCCCCAAGACCGGGCTTGTGCCAGTGACTCGATCACACAGGTGCCCAAGTTTAGATCTTTTGTGATGGATCCCAGGTCTCAGAAGCCGGGGTCTGTGTGAGCATCCCCATCCTCAGGACGGCTCCCAACGTTGGCCCTGCAGCTTCCAGAGATGGGGGTGACAGCAGCTGCTGTGCTGGTGGGAATTTTAATTTTTTTAAATTTTAAGTTCTCTTTTGATTTTAATTTTTTAAGAAGTTAAAGTTGTGTATATTTACTGTGTATGACATGATGTTTTGAAACACATATACATGGCAAAATGATGATGGCATTCAGGCTAATTACCCATCCATGACTCCTGGAGGGGATTTTAATGTGAAACTGTCCCAGAAAACCTCCCTGGCTTCTGGAGAAGATGCCTTCTTAGAGAAAGCACATCCTAAGAAAGTCTTCCAGAACTAGAAGTGGACTCTTCTGCCAGCACCTCAACAAGGCTTCCTATCCTTCCCTTTATGCTGGGAGCAGGTGGCAGAGCCTTGGGGACACTTCGTGCCTCCTCCCAGGAAGAGGCTCTGGGCACATTCCTCATCCAGCAGTTTTTCCTGGTGATGCATGGGCCCCTCTGCTGACTCCGGTCTTGGAAGCCGGAGACTGTGTGAGTGCGGGCTCCGGTAACACTCATGGTCCTCAGCTTCTGCTTTCCAGGGCTTGCTTCACCAGGAGGGACTCCATGTTGAATGCGTGGCCTCTCCTTGTGAAAAGCTGCACTTTCGGAAGCTGGTCCACACCAGCCAATGGGGCCGGGACCTCCTGCTGTGATTCTGGGCCTTCTTTCCCAGAGTTCACCTAATTTATCACAAGTTATTTTTGCCTGGAAGCCTGAGAAATCCTTTTACAGTGATAATTTCACTATGGCCACCTCTATCTCTGCAGCCTCCAGGCCTGCCTGGTCATCAAAGCCCACCTCATTGCAGATGGTCACCTAAGCCCACCTCATTGCAGATGGTCAGCTAAGCCCACCTCATTGCAGACCCAAAGCCTCCACCTCATTGCAGACCCAAAGCCTCCACCTCATTGCAGATGGTGAACTTTGTCATGATGCAAATTTTGCCACGTTCCTCTCCTGCCTAAAATCCTACAGTGGTTCTCCACTGCATTAGGTCAACAAATATTTACTGGAGACCATGACTGTTCTAGCCAGCAGGGATACATTGGTGGACAAAGCAGAGAAGCATCCCTGCCTTGTAGAGACGACATTTTCAGGGAAGTCAGACACCAAACAAGGCATAAGAAAACCGCAGGGCGTGTTGGAAGACTCATGTAGTGGGAGGGTGGACCTGGACACGGGAACCCGGAGGCCTAGGGGATGGGCACCGCAGAGTGGAGACCCCCCTGAGAGGTGGCGTTTGGACAAGGACAGAGAGTGAGGGAATGGGCCCATGTGTGTCTGGGGAGATGGCAGGTGAAAACCAAGGTGGCACCCACGTGGCTCCTGGAGGATGTTGAGCCAGGTCAGGGCAGTGAGAGGAGATGTGGTTGGAGGGGAAGCAGGGAGCCAGCCACACAGGGCCTGCACTTGGGATGAGGGGTGTGGCCTCCAGCTCCAGGTCCTGCCATTTTCTTCTCCTTTTTATGTTTCACCATCTTGGCCCTTTAGTTTCTTAAAGGTGAAAAGCCTTCCCAAAGGGGTTCCCACTTCCTGGAATTTTCTTGCTCTCCTGTCTGGCTCACACCCAGCATCCCTTTTTCCTGAAGGCCTTTCCTATCTCCCCCTCAGGGTGGTCCTCCTGCTTTACACAGCCTCAGTCAGGATGCCTCCTTCTCAGCTCTCTATCTGTATGCTAAATAGATGGATGGATGAATGGATGAGTGAACTGATGGATGGGTGGATGGGTGGACAAATGGATGGGTGGACAGGTGGATGGGTGGACGGGGGATGGGTGGATAGAGGACAGGTGGATGGGTGGACGGGTGGATGGGTGAACAGGTGGATGGGTGGACAAATAGATGGGTGGATGGGTGGACAAGTGGACAAATGGATGGGTGGACAGGTAGACAGGTGGACAAATGGATGAGTGGACAGGTGGACAGGTGGGTGAGTGGACAGGTGGACATGAGGGGGGGTGGACAGGTGGACAAATGAATGGGTGGACCAGTGGACAGGTGGACGGGTGGACAGGTGGATGGGTGGGTGGCTGGGTGGACAGGTGGAAAAAATGGATGGGGGGATGGCTGGACAGGTGGATGGGTGGGTGGATGAACAGGTGGACAAATGGGTGGGTGGACAAGTGGATGTGTAGATGGGTGGATGAATGGATAGGTGAACAGGCAGATGAATAGACAGGTGGATGGGCAAATGAATGGGTGGACAGGGGGTTCATTTACTTGATTTCTGCCTTCCTGACTGGACTGGAAGCTCTGTGAGGGGAGGGGCCCTTCTAGTCCACCTCTGCATCTCCAGAGCTTGAAATCGGTGGGCTGATTTCTGCTGATGGACTGACTGGTGCTGTTGATGGCGTGACTCAAGGAACAAAGGTTCTCAGGTTAGTGCTAGGATCCCAGACTGGACAACAGTCGGGTGAACGCTTCCCCAGGGTCAGTGAAGGAGGAAGCATGATAACGTGTGTAAGATCTGTGACTAGTTCTATAGTTCTATAAACTCTACAAAGCTGCTAATTATTATATAACTGGAAAATATCTATAGCACTTATGCTATACCAACAAATTATGTGCATTTCTACAATTGCCACTATAAAGAAATTATATATTAATACCTGCTCCTGGGACTCAATAGTATGATGGCTTTGAAATTAATACAGCAGCACAAATATCTCCTCAGAAAGACCTTTCCTTGCCCTCATCAAAATCGTCCTTCCCACAGTGCCATCTCATTACTGTTTCGCTTTCTCTCTGGCAATTACTCCTGCTGAAAATGACCATATTTACTTCTTTGCTCGTGAGTTCCGGGATGGCCAGTCCGCCTGGCCTGGAGTGCGGCTTCCCAGGTAGACACAGGAAGGAGTCACCTGGATTGCTCCTTCCTGAATTCCATCATTGAGAACGCGTTACCGTGGGTGGGAGTCAGGGACTGACAAAGTCAAGTCTTGCCAGTTTATTTCTAATTTATATAAGTGTCAAGAGCCAACATGAGCAACTGAATTATAGGTCAGAAAAAGATAATATCAAGAAAATATCCCCAAGATTAGTTTTAATGGTGTGAGTGAATAAATTAGTGAGCAGAGTAAAATGAAATTATGGGAAGGGACTGGGCACGGTGGCTCACGCTTGTAATCCTAGCACTGTGGGAAGCTGAGGCAGGAGGATCATTTGAGCCCAGGAGTTTGAGACCAGCCTAGACAGCACAGCAAGATCCCATGTCACAAAAGCAACAATTAAATTAAAAATATAAAAAGAAAAGAAATGATGGAAGGGGCTACGTGGCATTAACTACATATTGTTTCATTCTAAAGAACTTAATACAATCAATGTATACCTATATTTAGGAGACATTTCTCACACTCATGCCGTGTTTCAGTGGACACTCTCTCATGACAAGCTTTAAGAAAAAAAAGTCTCATAATAGCCTGAATGTATCTGTATCCAGCATAAACAGAAAAGGCGGAACAGTGATCAGGTTGTCTCTGAATGAGATAGGGCCTTTCCCAGCTTCTGCATGGACGTGCAGCCCAATATACACCAAATTAAGAAAACAGGAATGTGCCCTGGAGACGCGGGGAACAACACCACCCTCACCCTCACCCCAGAGACGTGGGGAACAAAGCCGCCCTCGCCCTCACCCTCACCCCAGAGACGTGGGGAACAACGCCGCCCTCACCCTCACCCTCACCCTCACGGCAGAGACGCGGGGAACAACACCACCTGCACCCTCATGCCTCAGCTAACCCTAAATTAAGAGCTTGCTGTGGAGACTTCAGAATCATTTCACTGTATACAGATTCAATGCTCAGGGGTTTAAATGGCATTATTTTTTCCACATACTGGACCTTACCTATTAATATTCAGGCCAGGAACAGCTTAAAATTGCAGAGAGCAAGCACACTAGCCAATAGCTCAGTGGGGAGCTGCGGGTAAGTAGCTGCCAGTCACCAGACCCAGGCCATTGTGAGCACAAGGGCCCTCACAGCAGCCCGACGGAGCCCCCGTCACTCGCAGAGCTCTGCAGGCTTGGGGGGCTGTGGCGAGGGAGGGGGAGGCCGGACCCAGCACAGGAAGAGGGGAGGTGAGGCGAGGCCGGGATGCCCTAGGCCACGCCAGCAGCAGGGACAAGCCCAGGGCAGGCAGGAGGCCCCGGAAATGCCCTGGGAAGGTACCACCTCCCTTCTCCTCCCCCCAGACCCCACTGCGGAGACCCAGCTTTCTGGGGACGCAAGCAGGGTGGCGCATCACAGACAGCGTGCTAGGAGAGTCAGCATAGCTGGGCCTGTTTATCCCGCTCCGTGGGCCAGGTGTTCTTGTCAGGGCGGCCGGTGGGCACGTGGGCCGGGCTGGTTGATGTGAGGGTGCGCCAAGCCCGCCTTCTCACCCAGGCATCCCCGACATGGGATCTGCCCAGCCTGCCTGGCAGTTTTCACTGGGAACATATTGTCCCCATCGTCGCTTAACTACGACTGGCGGGACCATGGGGCTCTGCCGGCCCAGCCCTTCCAGTGATGACGATGCTCCAATGGCCGTTCTCCGTGTCAAGCACCGGACGGCCCACCCTGCGACTCCGTCTCTGCGAGGCACACGCCATCTCCCGCCGCTCCCACTAGGCAGGGAGGGCCGAGCTGCTCCGGACGTATCTGCTGCCTGAGCTCCCTTGGGGCATAGTGGCCAAAAACAACCCTACACAGAGGTGAGGTCTTATCACAAAGGATAACCAATACCATATTTTTTCTTATGACAGCAATGACAATGACAGTGTACGTTCTACCCACAATGACAGATGTCAGCTGGGCTGCCACGTCTTAAGCAGATGTCATAAAATCAGAAAAGCATGGAGTGGCTGAAAGGGCGAGGGTGGCAGAGATGCTTGGGGCCTTTCTCTGAGCAAAAGATGTGGCCTCGTCCTGGAGAGGTGGGAGGGGAGGAGGCAGGTGTATGGAAAGCAGCCAACTGGCAAGAACAGGGTGGCTGCAGTCCTGGCCAGCAGATCCCGGCCACCAGGAGACTCTGAGCACAGGGCAGACACCGTGTGCAGGATGCCATCTCTCCTGCTGGTGAACATCCGGAGGACCTCTTGGCCCCCAGGGGCTGCACAGATAGGAAATATGCATAGATCTGAAAGGCTTTGGGACACATGTGAGGGACAGGGTCATCATCTGCTACCACAAGCAACAACAATATTCCTTGCCATTGAGTCGGTCTCTTTCTCTAGAAAGTAAATTCTCAATACGGGAAAGATTCCCTCCAGGGTGTTTACGGATGGTTGTGAGGGGACAGCTTCGGGACTTCTTACAGTTGCTCTAGCCTTTGTCCTTTGATTTCTTCACTTATTATAAATATGTCTTGAAAGCCTCGGGCTGGATGAAGAGAGGGGAGGGAAACACAGTCCCATCCTCACAGGATCCTTTTAGAGTCAGGCAGACAGTGTGGCAGTCAGCAGCCTGAGGTGATCATTTTCGCTGAAATCTGAATTAAAATTTCACTCGATTAAACATGCCTTCTTCATTTTGAGTAGCCACATTTGAAGTACTAAAGACCCACCCGGGGCCCGTGGGTGTGGTATTAACCAGGACGGACATGGAACAGGAATATTTCCACCGTCACAGGACGTTCCATCAGACGGCACGATTCCGAGTGTATGTGTGTTTTCAGCCACCAGGAAAACAGGCAGAGAAACCACCATGCACTGTGGATGCTCCCCCACCCCCCACCCCCAAGGCCCCTATGGACCCTCTTTGGGATCACTCTTTACTGCTATGTCCCAACTGAACACACACCTCAACACCTCCCAAATCCACACACCCACATGCGCAGACACACACGTTGCCACAGGAAAAGCATGATGTGGTTTATGTAAACCACCTTCTCATGGCGTTATACCTCACATGTGACTCTGGGAAAATGCACCTGCACTAACAGGACACAGTGATTCTATCGGGGATGAGATGGCCCGCACGGGCTTGAGGGGTTCGGTGCCATATTCCCCTCCTTCCTCCCCCACCCCTGGTACCATTGGTCCTCTTGCTCTGGGGTTGAGTGGGCATCTATGATGTGGTGTGTCTTGTCCTCCTGGAGGATCTGGGTGTGGTGTCAGTGCTAATGTCCTGGTAAGGATCATGTTCATTCTGGAGCCAGAGGCTTCAGTTCAGATCCTGGGTCATCTACCATCTATGTGACCTTGGAAAACTATTTAGCCTCTGTGAACCTCGGTTTCCTTATCTGCTCATCAGGGCTTGTTATTATTTTTATACACACCCCTTAGAGTTCTGATGAGGATTAAGTGAGCTAATATGTACCAGGCAATAGAAGAGCTCCTGGCAAATAGGAGTCATGGAATGCATTTTACTTATTGCTATTTGGGACAAAGACTCAATCTCATATTTTTTATACTCTTCATGTCGCTTGGCAGTTTTACTGAGTCGATGTCAGATTACTTAAAAATAATTTTTACTTAGCCTTAGATATGTTTTATATTTTTATCTAATTTTGTCTCCAATCTGTGTATTAAACTAGCTACCTCCAGCTACTGTCTCTATGTACCTGCCATGCTTCAAACATCCACACTGGTTTCAAATCTTTCCACATGTTTTTCTCTCTACTTGGAAAGACTTTTCAATCCTTACCTGACTTGCAAACTCCTGTACAAGCCTTAAAGCCCAGTTCTAAAATGACTTCCTTGAGAAAATATCACTGATTACCTAGGGCAGAAGGGATAGCTCCTTCTGGAATATTCCTATACATCTGTTCAAGCTGCTTTTAAATTATGTGCATCTTTAGTATCAATTTACATGCAGTTCTCCCCCTCCACTGGGAATCCCTGAACTAAGAATACTATCCATCCCACCGTGGCCACTCAGGAGATGTTTAGAATGATTGATATTGAATATCGAAGTTCAGAGATGGTGCGGCAGAGACCCATGGGTGGCACTGTCATCAGTGAGGTCTGGGAAAGACCAGAAGGCATTGAGCTCCTTGCTGCTGGGTCTCCCAGCTCCTTCTCTAGCCCTGGATTGTCTGCCCTGCTTTCCCATGCAACATCCCATGCAGTTTGTTCATTTTGTGGCTGTCTGCAGACGTTTAGGGATATGCAGAGGGGGCTTTAAGCCCTCAGCAAGGAAGAGAATATAGGTCCAACTGACAAGGGTCATTTGCTGAAAGTGAAGTTAATAAAAATCGGCCTCAGACAGGAGAGACTGACCCCCTGCAGAAGGAGCTCCCTCCAGGCAAAGCCAAGCTGGTTCATTCCTCCAAGCTTCTGTTGGGCGTTTCTGCCTCAGACACTCTGCTTAGCACTGAGGAGACGCTGGCACTAGGACGCAGCCAGCCTCTGCCCTCAAGGAGCTGACAGTCTGTTTCGGAAATCTCCAAGGGTCCTAACCCTCTATCTGCATTTCTGAGGCCCGGAGGTCCTGAGAACAAAGACCTTGCTGTTCATTCGGTGGCTGGTGATGGGGTGTTGCGTGTCTGGGGTCCGCGCCAGCTGACCACTCACTAGGCAGCAGAACCTCGCTGAACTGTGGAGGCTATTTCTAGTCTATACCCCAGTAAATGTGCACGCCTGACATTTTAACATGGAAATATTGGTGTGTTTGATTTTGAAGGGTCGTTCAGGCCCTGTGCAATATTTGTAGCCAATGTATGCATTCTTCTGAAACCTGACAGATTCTGAACACATCTGCCTATGGGGGTTTTGGCCAAAGCCTGTTGGACTTGGAATCAGGTTAAGTTTTAGGAATATCTATCAAGGACACCAGAGTGTGTCCTGAAACCAGTCAAGATGAGAGAGGTGGGCCGGAAGCGATGGCTCACTCCAGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCAGATTACCTGAGATCAGGAGTTAGCCTGGCCAACATGGTGAAACCTAGTTTCTACTAATAATACAAAAATTAGCCAGGCATGGTGGTGCATGCCTGTAATCCCAGCAACTCAGGAGGCTGAGGCAGGCGAATTGCTTGAACCTGGGAGGTGGTGGTTGCAGTGAGCTGGGATCGTGCCACTGCCCTCTACCCCAGGTGACAAGAGCAAAACTCCATCTCAAAAACAAACAAACAAACAAACAAAACCAACCAACCAACCAAACAAACAAACAAACAAAAAAAGAGAGGCAAGATGGCCCCAGGAAACCAAAGGTCATGGTACCATGTAAATAACACAACCACCTGAAGATGGCTGCAGCCCAAGGGACTGTGTTTTCAGACACCATGGCTGCGAGAAGAACAGAGTGGGACAGGAGGGAGACACCACAGCACCCACTCCCCTCCGGGTACCCACTCCCCTCTGGGTACCCACTCCTCTCCGGGCACCCACTCCCCTCCAGGCACCCACTCCCCTCCGGGTACACACTCCTCTCCAGGCACCCACTCCCCTCCGGGCACCCACTCCCCTCAGGGCACCCACTCCCCTCTGGGTACCCACTCCCCTCCGGGTACCCACCTCTCTCCGGGCACCCACTCCCCTCCGGGCACCCACTCCTCTCCAGGCACCCACTCCCCTCAGGGCACCCACTCCCCTCCGGGCACCCACTCCCCTCCAGGACGCTCCAGCGTCTGGCTTCTGGACGTGTCCACTGCTGAGTGTACACAGGCCTCAGCTCCCACCTGGGCAAACATCGCCTGGGCAGTCGTCCCCTGTGCCAGTCCCAGGACCCAGGGCCCTGTGTGCTGCTCCTCACAGGCGACCTTCTGCCTACCGCCTGTGAACAGCCAAACCAGCTGGCTGGAGGCTGGGAGAACAGGGCCCCGAGGCTGAGTCAGCCTGGGGTCCCAGCCAAGATGCAGATGCGGAGAGACCCAGGCCCCCCGCAGCTGACCAGACACGTGAGAGGGGCAGCCGGGCCCAGCGGACCCTGAGCCCATCCCAGGCCACACTGCCCAGGCACAGAGCGTGGAGCTACATAAACAGGCGTTGCTTTAAGCCCCTAAGGTGTGGAGCAGCAGTGCAGTTGGAGGCACCCTGTGTGTGCTCACAGATACAGGCGGAATCAGGCAAGGGCTGCTGGGAAAGAACGGCAGGGCCACATCGGCCTCTGCGGGACAAGGACACCGGCTCCCGATGCCTGAAAGTGCCTGCACCAGCCTCACCGTGCACTCTGCCTCCAAGAGCAGAGAGTGTCTCGCAACCACATCGCTTCCTGGCTACGCCGTTCTGCAGGCTGAAATGGCTTCCATTCACCCAAGCACAATTCCTGGAGAATCCTGAGACACACGAGGCTCACGTTTTCTCTATGTATCTAATTACCAGGAATAATGAGGCAGGGAAACCTGAACGGTTGTGAATTATCCCACAAGAAGACGTCCACGGGCAGAGGCCACCTGTCGCAGGGGAAGGCAGGCAGCTGTCCCTGGGAATGACACACAGCCCACCTTCTTTCCCTCGGCTCCTCGGTGTGTTAATCTGATGTGTCTGTTTCCCTCTAAAGGTTTTGTTCTTTTCACACTGCAGATGGATGTGCAATATGCCGCTTTTGCACTAGAGGCAAGAGAATCAGGTGGAATCTCACAGCACTTCTCCCGGACTCCTGGGCCGTCTGCACCACGTCCAAGTCAAGGGCCCTGGACTGAGGCACATGCCCACTTCCTGCGTCTTTGCGATAAAATGAGTGTGCGGTTCACACTCACTTCCTGCATCCCTGAGGTGAGGCTGTGGTGGTGAGGCCGTGGTGGTGAGGCCGTGTGGTTGAGGCCGTGGGGGTGAGGCCGTGGGGTGAGGCCATGGGGTTGAGGCTGTGGGGTGAGGCCGG
     READ_BASE_COUNTS: 6282, 6408, 6286, 6163, 0
          READ_FILTER: SRA_READ_FILTER_PASS
             READ_LEN: 25139
           READ_START: 0
            READ_TYPE: SRA_READ_TYPE_BIOLOGICAL
          REPLACED_BY:
             REPLACES:
     SEQID_GNL_PREFIX: WGS:JTFH01
               SEQ_ID: gi|725026265|gb|JTFH01000001.1|
           SEQ_ID_GNL: gnl|WGS:JTFH01|decoy00001
           SPOT_COUNT: 1998
           SPOT_GROUP:
              SPOT_ID: 1
             SPOT_LEN: 25139
               STRAIN:
    TARGETED_LOCUS_ID:
  TARGETED_LOCUS_NAME:
                TAXID: 9606
                TITLE: Homo sapiens isolate Pool of 254 public SGDP samples decoy00001, whole genome shotgun sequence
             TRIM_LEN: 25139
           TRIM_START: 0

klymenko commented 1 month ago

Run vdb-config -i; Uncheck "Enable remote access", if it is enabled (type 'r'); type 's' to save; type 'x' to exit.

Then run again align-info path/SRR22287940 and post here the output.

sa2adeel commented 1 month ago

JTFH01000001,chrUn_JTFH01000001v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000002,chrUn_JTFH01000002v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000003,chrUn_JTFH01000003v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000004,chrUn_JTFH01000004v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000007,chrUn_JTFH01000007v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000008,chrUn_JTFH01000008v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000012,chrUn_JTFH01000012v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000013,chrUn_JTFH01000013v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000015,chrUn_JTFH01000015v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000017,chrUn_JTFH01000017v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000018,chrUn_JTFH01000018v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000022,chrUn_JTFH01000022v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000025,chrUn_JTFH01000025v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000026,chrUn_JTFH01000026v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000029,chrUn_JTFH01000029v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000031,chrUn_JTFH01000031v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000033,chrUn_JTFH01000033v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000039,chrUn_JTFH01000039v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000040,chrUn_JTFH01000040v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000044,chrUn_JTFH01000044v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000045,chrUn_JTFH01000045v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000050,chrUn_JTFH01000050v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000054,chrUn_JTFH01000054v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000059,chrUn_JTFH01000059v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000060,chrUn_JTFH01000060v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000061,chrUn_JTFH01000061v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000063,chrUn_JTFH01000063v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000064,chrUn_JTFH01000064v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000068,chrUn_JTFH01000068v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000070,chrUn_JTFH01000070v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000071,chrUn_JTFH01000071v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000072,chrUn_JTFH01000072v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000077,chrUn_JTFH01000077v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000079,chrUn_JTFH01000079v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000087,chrUn_JTFH01000087v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000089,chrUn_JTFH01000089v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000090,chrUn_JTFH01000090v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000092,chrUn_JTFH01000092v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000096,chrUn_JTFH01000096v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000098,chrUn_JTFH01000098v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000101,chrUn_JTFH01000101v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000107,chrUn_JTFH01000107v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000108,chrUn_JTFH01000108v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000110,chrUn_JTFH01000110v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000111,chrUn_JTFH01000111v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000112,chrUn_JTFH01000112v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000113,chrUn_JTFH01000113v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000114,chrUn_JTFH01000114v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000116,chrUn_JTFH01000116v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000117,chrUn_JTFH01000117v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000118,chrUn_JTFH01000118v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000119,chrUn_JTFH01000119v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000123,chrUn_JTFH01000123v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000124,chrUn_JTFH01000124v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000126,chrUn_JTFH01000126v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000127,chrUn_JTFH01000127v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000129,chrUn_JTFH01000129v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000130,chrUn_JTFH01000130v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000132,chrUn_JTFH01000132v1_decoy,false,remote:path/SRR22287940/JTFH01

klymenko commented 1 month ago

Try to run fasterq-dump or fastq-dump of /cluster/projects/hopegroup/dbGap/prj_35579/SRR22287940 again. Does it fail now?

sa2adeel commented 1 month ago

Still fails with fasterq-dump.

2024-07-13T18:58:29 fasterq-dump.3.1.1 warn: directory not found while opening manager within virtual file system module - can't open JTFH01000001 as a RefSeq or as a WGS
2024-07-13T18:58:29 fasterq-dump.3.1.1 err: sorter.c producer_thread_func: rec.read.len = 0
2024-07-13T18:58:30 fasterq-dump.3.1.1 err: sorter.c run_producer_pool().join_and_release_threads -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)
2024-07-13T18:58:30 fasterq-dump.3.1.1 err: sorter.c execute_lookup_production() -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)
2024-07-13T18:58:30 fasterq-dump.3.1.1 err: fasterq-dump.c produce_lookup_files() -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)

=============================================================
An error occurred during processing.
A report was generated into the file 'usr/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fasterq-dump quit with error code 3

klymenko commented 1 month ago

I will need to debug it on Monday.

klymenko commented 1 month ago

What ngc file did you use to prefetch SRR22287940?

sa2adeel commented 1 month ago

The ngc file was downloaded from the dbGap repository that is specific to our groups data request application. It was the same file used to download the other accessions. I am not sure if I can share the file.

klymenko commented 1 month ago

Don't share it. What is project's number?

sa2adeel commented 1 month ago

project # 35579

klymenko commented 1 month ago

How many files do you have in SRR22287940/ directory?

sa2adeel commented 1 month ago

There are 592 files in total.

klymenko commented 1 month ago

What is the output of the following? align-info /cluster/projects/hopegroup/dbGap/prj_35579/SRR22287940 | wc -l

sa2adeel commented 1 month ago

I get 1552 back.

klymenko commented 1 month ago

What is the output of the following? ls -l /cluster/projects/hopegroup/dbGap/prj_35579/SRR22287940/SRR22287940*

sa2adeel commented 1 month ago

-rw-r----- 1 usr gdc 1278275591 Jul 8 16:05 SRR22287940/SRR22287940.sra

klymenko commented 1 month ago

It is a bug, thank you for finding it, we will fix it in our following release.

Currently run fasterq-dump from the same directory where run directory SRR22287940/ is located (/cluster/projects/hopegroup/dbGap/prj_35579).

Use --outdir argument to specify directory where to write output files.

sa2adeel commented 1 month ago

Awesome, thank you!

ncbi / sra-tools

fastq-dump/fasterq-dump: can't open JTFH01xxxxxx as a RefSeq or as a WGS #946