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ncsa / NEAT

NEAT (NExt-generation Analysis Toolkit) simulates next-gen sequencing reads and can learn simulation parameters from real data.
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Paired reads orientation generation behavior #24

Closed MeatKatlet closed 3 years ago

MeatKatlet commented 3 years ago

Hello. I have noticed wrong behavior of paired read simulation. Read2 when orientation==False equal to read1. This also seems to be related to https://github.com/ncsa/NEAT/issues/17 issue.

Since if write_fastq_record() function must simulate random(50/50) Illumina adapter ligation as described for example here or here I change write_fastq_record() function accordingly to this behavior.

Thank you for maintaining this project.

Kirill.