ndaniel / fusioncatcher

Finder of Somatic Fusion Genes in RNA-seq data
GNU General Public License v3.0
141 stars 66 forks source link

ERROR: Too long read sequence found. #204

Open li1ba opened 1 year ago

li1ba commented 1 year ago

Hi!

I'm trying to run FusionCatcher v1.33 and I get an error:

////////////////////////////////////////////////////////////////////////////////
  Running: step = 78   Time: 12:53   Date: 2022-09-17 (elapsed time: 0d:0h:7m)
\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
==> Current working directory: '/opt'
fastqtk \
lengths  \
 /datafiles/out/init-1_blox-0_2.fq \
 /datafiles/out/log_lengths_original_reads_r_0.txt
--------------------------------------------------------------------------------
+-->EXECUTING...
ERROR: Workflow execution failed at step 78 while executing:
----------------
   fastqtk \
   lengths  \
    /datafiles/out/init-1_blox-0_2.fq \
    /datafiles/out/log_lengths_original_reads_r_0.txt
----------------
  * Size '/datafiles/out/init-1_blox-0_2.fq' = 16800589587 bytes
  * Size '/datafiles/out/log_lengths_original_reads_r_0.txt' = 0 bytes
Executing second time the same step/command in order to capture error messages (i.e. STDERR)...
-------------------------------------------
ERROR: Too long read sequence found. Maximum length of supported reads is 32768.
################################################################################
################################################################################
TOTAL RUNNING TIME: 0 day(s), 0 hour(s), 7 minute(s), and 0 second(s) 
################################################################################
################################################################################

I checked both FASTQ files with FastQC and there are no reads longer than 101bp. Could you please help to solve this?

Thanks!