ndaniel / fusioncatcher

Finder of Somatic Fusion Genes in RNA-seq data
GNU General Public License v3.0
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Error downloading Canis lupus familiaris genome from Ensembl #221

Open SeungWWoo opened 2 months ago

SeungWWoo commented 2 months ago

Hi,

I encountered an error while trying to download the genome of Canis lupus familiaris from the Ensembl server using this commend. fusioncatcher-build.py -g canis_lupus_familiaris -o genome_build

As a result, it fails to find the genome and mitochondria files and repeatedly displays an error message.

fusioncatcher-build.py -g canis_lupus_familiaris -o genome_build

get_genome.py \
--organism canis_lupus_familiaris \
--server ftp.ensembl.org \
--output <my path>
--------------------------------------------------------------------------------
+-->EXECUTING...
Downloading the genome of organism 'canis_lupus_familiaris' from Ensembl!
230 Login successful.
ERROR: Cannot find the genome and mitochondria files!
['Canis_lupus_familiaris.ROS_Cfam_1.0.dna.toplevel.fa.gz']

ERROR: Workflow execution failed at step 4 while executing:
----------------
   get_genome.py \
   --organism canis_lupus_familiaris \
   --server ftp.ensembl.org \
   --output <my path>
----------------

Executing second time the same step/command in order to capture error messages (i.e. STDERR)...

-------------------------------------------
Downloading the genome of organism 'canis_lupus_familiaris' from Ensembl!
230 Login successful.
ERROR: Cannot find the genome and mitochondria files!
['Canis_lupus_familiaris.ROS_Cfam_1.0.dna.toplevel.fa.gz']
################################################################################
################################################################################
TOTAL RUNNING TIME: 0 day(s), 0 hour(s), 0 minute(s), and 7 second(s) 
################################################################################
################################################################################

The reference genome appears to lack the mitochondrial (MT) sequence, which is required for FusionCatcher to function correctly.

In this case, am I unable to use FusionCatcher?

If you have any solutions to resolve this issue, I would greatly appreciate your assistance.

Any help is appreciated.

Thank you.