search

ndierckx / NOVOPlasty

NOVOPlasty - The organelle assembler and heteroplasmy caller
Other
178 stars 71 forks source link

invalid seed #149

Open sha1987-sss opened 4 years ago

sha1987-sss commented 4 years ago

hello，I am trying to use this software to assemble the chloroplast genome, and the result shows INVALID SEED, PLEASE TRY AGAIN WITH A NEW ONE. I want to know where mistake happened, DoIyou have any suggestion?The following is my log_Test.txt

NOVOPlasty: The Organelle Assembler Version 3.1 Author: Nicolas Dierckxsens, (c) 2015-2019

Input parameters from the configuration file: Verify if everything is correct

Project:

Project name = Test Type = cholo Genome range = 130000-170000 K-mer = 39 Max memory = Extended log = 0 Save assembled reads = no Seed Input = /w/user249/data4/cleandata/Seed_RUBP_cp.fasta Reference sequence = Variance detection = Heteroplasmy = HP exclude list = Chloroplast sequence =

Dataset 1:

Read Length = 151 Insert size = 300 Platform = illumina Single/Paired = PE Combined reads = Forward reads = /w/user249/data4/cleandata/Nitella_FDSW202226034-1r_1.clean.fq Reverse reads = /w/user249/data4/cleandata/Nitella_FDSW202226034-1r_2.clean.fq

Heteroplasmy:

Heteroplasmy = HP exclude list = PCR-free =

Optional:

Insert size auto = yes Insert range = 1.9 Insert range strict = 1.3 Use Quality Scores = no

Subsampled fraction: 100.00 %

Retrieve Seed...

INVALID SEED, PLEASE TRY AGAIN WITH A NEW ONE

ndierckx commented 4 years ago

Hi, Could you first try again with the latest version, because this one is very old... And make sure you download the config file fro the correct version, they are not matching So just redownload the latest version and start again Also you put as type "cholo", it should be "chloro"