Open AndriiTarieiev opened 1 year ago
You mean you are getting different assemblies depending on the given k-mer?
No, the assemblies with different k-mers are actually very similar. However, when I align them between each other and to the reference, there is a shift of initial/starting position (where the circular genome is split to linearize it) on ~23-75kbp (shift increases with increasing k-mer size).
To make the problem a bit more visual, I prepared the following illustration based on one real sample: NOVOPlasty_plastidassembly&_alignment.pdf
Hi, I do not understand the issue, does it matter where it starts, it is a circular genome, there is no starting point. Starting point will depend on at which time in the assembly process both ends connect with each other.
Dear developers,
I'm experiencing significant shifts of initial/starting point in comparison to the reference sequence when doing plastid assembly using NOVOPlasty. These shifts are dependent on k-mer length. I would appreciate it very much if you are able to suggest any solution to this problem.
Best regards, Andrii