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ndierckx / NOVOPlasty

NOVOPlasty - The organelle assembler and heteroplasmy caller
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Input file format #221

Closed lightworms closed 5 months ago

lightworms commented 6 months ago

Hello I'm receiving the following error:

THE INPUT READS HAVE AN INCORRECT FILE FORMAT! PLEASE SEND ME THE ID STRUCTURE!

version: 4.3.3

Forward:

@ERR104974.12 HWI-ST427_0113:1:1101:1265:2704#GNNGNGNG/1 AGGGGATCATGGCAACATACAATCTAATTATCACCGATGTTACGCAGTACGGCAACTCATACTACGGCATCCGATATTGCGTAGCTGGATGGGACATCGAT + HHHHHHHHHHHHHFFFHHHHFHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHFHHHHHHHHHFHFGHHHBFGGGGHBEEHBFF;FCDD>.00;=7E @ERR104974.13 HWI-ST427_0113:1:1101:1471:2707#GNNGNGNG/1 CTTTTTGCTGGTCCATCACAAAGAGCCCCGTTCCGCATATCACAAAGACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGCGATCTCGTATGCCGTCTTCT + HHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHHHEHHHHHFHHHHHHHGFHHFHHHHFHHEHFHHHFHHHGGHGG>5.ABC?;FGDGGFHHFH6

Reverse:

@ERR104974.11 HWI-ST427_0113:1:1101:1453:2688#GNNTNANG/2 TACTGGCGATCCTAATTATAAGATACAATTTCTAATTCCAACGGTACCTATCTTATATTTAGTATCGCCAGTAAGATCGGAAGAGCGTCGTGTAGGGAAAG + HHHHHHEH;@FGHGBBCBDDGGFEGHHHGHBHHGFHHFCHFGFFBHHFHFGGHGHHHEFHFGHHFHHGGFHHHHHGFEFFA=DADCEAEE?A=BDB @ERR104974.12 HWI-ST427_0113:1:1101:1265:2704#GNNGNGNG/2 TTCCCTGGACCGACGTATTTCGAATCCCAGAAACGAGAAAGCTCAACTGCGGCGTTTGCCTCAAGCGGCTCCGGACGGATCATCCGTTTTCGATCGATGTC + HHHHHHHHHHHHFHHHHHHHHHHHHHHHEHFFHHHHHHFHHCFHEHHHHFHHHHFHHHHHHHFHHHFBFHFHFHBDFF,CEEEEEF@EFE:<CDCCCAE<<

All the best

ndierckx commented 5 months ago

Hi, these read ids should not be a problem, can you send me your log file? Are you sure you didn't switch the forward and reverse path?

lightworms commented 5 months ago

Thank you for getting back to me; here's the log file. log_ERR104974_Floridensis_Test.txt

lightworms commented 5 months ago

Whoops, the problem was that I was using a compressed version and an uncompressed for my forward and backward reads. Resolved!