Open 11malong opened 1 year ago
I am having the same issue. Is there any fix for this?
Hi, Sorry I recently moved to Japan and lost track of this issue.
This should be easy to fix, seems like the config file got corrupted and it doesn't recognize the numeric value 30 anymore.
Did you run it on Windows, Linux or Mac
And can you redownload the Sim-it perl file and try again?
I found the issue, by altering the config file in certain windows software, windows carriage return are added to the file, which is not recognized by my script.
But I added some lines to remove them, so they should not be an issue anymore, you can try again by redownloading the script
Hi, I have a question: my config.txt is like:
Project:
Project name = Test Reference sequence = /mnt/f/mshunter/chm13_1000000/chm13_after.5.fa Replace ambiguous nts(N) = Max threads = 8
Structural variation:
VCF input = Foreign sequences =
Deletions = 0 Length (bp) = 30-150000
Insertions = 0 Length (bp) = 30-100000
Tandem duplications = 0 Length (bp) = 50-10000 Copies = 1-20
Inversions = 0 Length (bp) = 150-1300000
Complex substitutions = 0 Length (bp) = 30-100000
Inverted duplications = 0 Length (bp) = 150-350000
Heterozygosity = 60%
Long Read simulation:
Sequencing depth = 30 Median length = 15000 Length range = 10000-20000 Accuracy = 95% Error profile = error_profile_PB_Sequel_CCS_hifi.txt
and my command is like: perl ./Sim-it1.3.4.pl -c ./config_Sim-it_my.txt -o ./output
but I got error like: --------------------------READ SEQUENCING DEPTH FILE---------------------------- , No such file or directoryr can't open sequencing depth file 30
How can I solve this? Thank you so much.