I would like to take a 35mm dish with HeLa cells, image it a bit in BrightStar, send deepthought information to EvaGreen, and find the same cells, and continue imaging.
Why is it useful?
A single sample can be imaged reliable across different imaging systems.
How is it done now?
This cannot be achieved currently, as finding the same region of interest is close to impossible for many reasons
coordinate system between leica and olympus systems are different.
there's no reliable way to find the same cells again after the established stage coordinates are changed. the problem becomes difficult when a completely different microscope has to observe the known sample.
Philosophy
I suppose, in some ways, we are figuring out how do we make the microscope recognize the sample, such that we can find the same region in two different systems. Or recognize one sample from another.
Problems
conceptualize how you'll track a biological entitiy that can have a new physical location because of either biological motility or stage movement
how will you evaluate if the known sample-stage coordinate transformational matrix is outdated? how will you update it?
pskeshu
commented
3 years ago
Application
I would like to take a 35mm dish with HeLa cells, image it a bit in BrightStar, send deepthought information to EvaGreen, and find the same cells, and continue imaging.
Why is it useful?
A single sample can be imaged reliable across different imaging systems.
How is it done now?
This cannot be achieved currently, as finding the same region of interest is close to impossible for many reasons
Philosophy
I suppose, in some ways, we are figuring out how do we make the microscope recognize the sample, such that we can find the same region in two different systems. Or recognize one sample from another.
Problems