Hi!
I just wanna check whether Kraken can serve my purposes!
I have a still unpublished genome assembly, which I used to map and annotate my DNA-Seq pop data.
I want to use the ref genome for the zebra finch available from ensembl as my ancestral ref in some pop gen analysis.
For this, would I have to convert the coordinates from the zebra finch available from ensembl to mine unpublished one.
Or would I have to realign the zebra finch to my genome?
I have my genome in scaffolds and the gtf file for it.
Thanks!
Hi! I just wanna check whether Kraken can serve my purposes! I have a still unpublished genome assembly, which I used to map and annotate my DNA-Seq pop data. I want to use the ref genome for the zebra finch available from ensembl as my ancestral ref in some pop gen analysis. For this, would I have to convert the coordinates from the zebra finch available from ensembl to mine unpublished one. Or would I have to realign the zebra finch to my genome? I have my genome in scaffolds and the gtf file for it. Thanks!