I am using TreeTime (v 0.11.3), installed with conda install bioconda::treetime.
I am using the ancestral sequence reconstruction (with VCF files) command, treetime ancestral --aln data/tb/lee_2015.vcf.gz --vcf-reference data/tb/tb_ref.fasta --tree data/tb/lee_2015.nwk , (but with my own files) as a means to obtain the branch_mutations.txt output, but am finding that this output is always empty. The other outputs (ancestral_sequences.vcf, annotated_tree.nexus, auspice_tree.json, sequence_evolution_model.txt) are not empty.
I am unsure of what is going on here, and was wondering if you could help?
Dear Devs,
I hope that you are well.
I am using TreeTime (v 0.11.3), installed with
conda install bioconda::treetime
.I am using the ancestral sequence reconstruction (with VCF files) command,
treetime ancestral --aln data/tb/lee_2015.vcf.gz --vcf-reference data/tb/tb_ref.fasta --tree data/tb/lee_2015.nwk
, (but with my own files) as a means to obtain the branch_mutations.txt output, but am finding that this output is always empty. The other outputs (ancestral_sequences.vcf, annotated_tree.nexus, auspice_tree.json, sequence_evolution_model.txt) are not empty.I am unsure of what is going on here, and was wondering if you could help?