Evaluate Bleaching on the movie

[zhouyi0812]

Hello, I want to see if my movie is bleaching and trying to get a graph for the mean project on each frames or in between certain frames. I am not sure how I could integrate with the mes platform. Or, how can I get the numbers for mean projection on each frame? Thank you!!

[kushalkolar]

Assuming dimension 0 is time:

array.mean(axis=0)

On Mon, Dec 4, 2023, 17:08 Yi Zhou @.***> wrote:

Hello, I want to see if my movie is bleaching and trying to get a graph for the mean project on each frames or in between certain frames. I am not sure how I could integrate with the mes platform. Or, how can I get the numbers for mean projection on each frame? Thank you!!

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[zhouyi0812]

So this code supposed to use after mc? mcorr_movie = df.iloc[0].mcorr.get_output() like this: mcorr_movie.mean(axis=0)

[kushalkolar]

Memmaps are arrays.

You would benefit from a basic tutorial on numpy: https://youtu.be/ZB7BZMhfPgk?si=wytdpIYIdQwn-JlI

On Mon, Dec 4, 2023, 17:44 Yi Zhou @.***> wrote:

So this code supposed to use after mc? mcorr_movie = df.iloc[0].mcorr.get_output() mcorr_movie.array.mean(axis=0) AttributeError: 'memmap' object has no attribute 'array'

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[zhouyi0812]

I think I figure out this and want to post my code here if someone needs it

frame_means = []

for frame in mcorr_movie: frame_mean = frame.mean() frame_means.append(frame_mean)

frame_means = mcorr_movie.mean(axis=(1, 2)) frame_means num_data_points = frame_means.shape[0]

print(f"Number of data points in frame_means: {num_data_points}") plt.plot(frame_means)

plt.title('Mean Intensity of Each Frame') plt.xlabel('Frame Number') plt.ylabel('Mean Intensity') plt.show()

[kushalkolar]

Yup that's right, sorry axis=0 gives the mean image.