Closed zhouyi0812 closed 7 months ago
gSig_filt has nothing to do with the noise level of the movie, I'm confused?
I might misunderstood... I thought the high pass filter decide the size and number of kernel of gaussian matrix --> the filter apply on the florescence intensity on individual pixel, and smooth the low level frequency information --> the bigger the filter the brighter the pixel but lose more detailed info --> if the noise level is generally high --> we choose a relative big number of gsig_filt
You want to choose a value that enhances - landmarks in the movie
Which means the function of high pass filter in this case doesn't do anything about noise reduction or impact the low level info? emm...if so what is the different between choosing 3 and 5 if they both enhance the landmarks Thank you!
If two different values both enhance the landmarks visually I would just try both and see which one works best, that's what mesmerize is for :)
The gSig_filt visualization helps with that.
closing due to inactivity
Hello! I wonder in the step of selecting the proper gsig_filt, if there is a way that can check the noise level for the original movie? And before and after applying the gsig_filt? Thank you!!