neurogenomics / MAGMA_Celltyping

Find causal cell-types underlying complex trait genetics
https://neurogenomics.github.io/MAGMA_Celltyping
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Add full AIBS data from all regions #138

Closed bschilder closed 1 year ago

bschilder commented 1 year ago

The ctd_AIBS.rds object was generated from an old version of the Allen Brain Atlas sc/snRNA-seq data that only came from temporal cortex. @Mike-robiology generated an updated CTD using all brain regions.

From Slack correspondence with @Mike-robiology

The data is Smart-seq from the Allan Brain Map of multiple cortical areas (here’s a link to a forum post with more detail). It includes the data from Conserved cell types with divergent features in human versus mouse cortex (2019), Comparative cellular analysis of motor cortex in human, marmoset and mouse (2021), along with some unpublished data. Here is the link to the actual data. The ‘Table of cell metadata’ and ‘Gene expression matrix’ files were used Attached is the script used to process it (AIBS_CTD_Michael.R). I’ve simplified the level_2 annotations from what I already described to match the style used in the current version of scFlow. This has reduced the file size to ~40MB.

AIBS_CTD_Michael.R.txt

bschilder commented 1 year ago

The file Michael provided is in our lab's Google Drive resources/EWCE folder. I've downloaded that file, standardized it, and uploaded it to the Releases:

ctd=readRDS("~/Downloads/AIBS_Michael_scFlow.rds")
ctd2=EWCE::standardise_ctd(ctd, input_species = "human")
saveRDS(ctd2, "ctd_allAIBS.rds")