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`useMicromamba` option doesn't work - generates Conda instructions instead. #4161

Closed jemma-nelson closed 2 weeks ago

jemma-nelson commented 1 year ago

Bug report

Expected behavior and actual behavior

According to the docs, the useMicromamba directive should cause Nextflow to use Micromamba instead of Conda.

When using this option, the nextflow process fails to run, because the generated .command.run file still uses conda.

Steps to reproduce the problem

On a machine with micromamba installed, but not conda:

git clone https://gist.github.com/jemma-nelson/921e3a8aa32efe22bd73fbe1cbdfd09b
cd 921e3a8aa32efe22bd73fbe1cbdfd09b/
bash run.sh

Program output

The .nextflow.log file, as well as the generated .command.* files are in the gist referenced above. (e.g: https://gist.github.com/jemma-nelson/921e3a8aa32efe22bd73fbe1cbdfd09b#file-nextflow-log)

Program output:

N E X T F L O W  ~  version 23.04.2
Launching `main.nf` [nostalgic_ardinghelli] DSL2 - revision: e1ee91cb36
[-        ] process > greet -
Creating env using micromamba: /home/nelsonjs/tmp/repro-micromamba/test-repro/921e3a8aa32efe22bd73fbe1cbdfd09b/env.yaml [cache executor >  local (1)                                                                                                          [e2/667fd3] process > greet (1) [  0%] 0 of 1
executor >  local (1)                                                                                                          [e2/667fd3] process > greet (1) [  0%] 0 of 1                                                                                  executor >  local (1)                                                                                                          [e2/667fd3] process > greet (1) [100%] 1 of 1, failed: 1 ✘                                                                     Creating env using micromamba: /home/nelsonjs/tmp/repro-micromamba/test-repro/921e3a8aa32efe22bd73fbe1cbdfd09b/env.yaml [cache /home/nelsonjs/tmp/repro-micromamba/test-repro/921e3a8aa32efe22bd73fbe1cbdfd09b/work/conda/base-bda312ce1312db01eb3e5e99160dbdb1]
ERROR ~ Error executing process > 'greet (1)'

Caused by:
  Process `greet (1)` terminated with an error exit status (1)

Command executed:

  echo hello world

Command exit status:
  1

Command output:
  (empty)

Command error:
  .command.run: line 119: conda: command not found
  .command.run: line 119: /bin/activate: No such file or directory

Work dir:
  /home/nelsonjs/tmp/repro-micromamba/test-repro/921e3a8aa32efe22bd73fbe1cbdfd09b/work/e2/667fd3bde76e5bdf8df569044e15a6

Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out`

 -- Check '.nextflow.log' file for details

Environment

Additional context

I mentioned this in slack last week, but did not get any attention. https://nextflow.slack.com/archives/C02T98A23U7/p1690325743801519

As near as I can tell from examining the source code, this configuration option cannot work, nor can the useMamba option, as nextflow always emits conda info --json for conda activation.

You may also want to add --yes to the micromamba create command, because if export MAMBA_ALWAYS_YES=true is not set, environment creation will hang indefinitely.

bentsherman commented 1 year ago

Fair enough, should be a simple fix. Fwiw, I've been using Mambaforge which provides mamba as a drop-in replacement for conda rather than a separate command, so that I don't have to bother with these settings.

pditommaso commented 1 year ago

Do you mean miniforge? Not sure it's the same it says

conda-forge set as the default (and only) channel.

In any case the the support for micromamba should be honoured as described in the docs

bentsherman commented 1 year ago

Mambaforge is miniforge with mamba as the default conda backend. Not sure why it says conda-forge is the only channel because it allows any channels.

pditommaso commented 1 year ago

Unable to replicate. Nextflow uses micromamba with the provided test case

Caused by:
  Failed to create Conda environment
  command: micromamba env create --prefix /Users/pditommaso/Projects/921e3a8aa32efe22bd73fbe1cbdfd09b/work/conda/base-bda312ce1312db01eb3e5e99160dbdb1 --file /Users/pditommaso/Projects/921e3a8aa32efe22bd73fbe1cbdfd09b/env.yaml
  status : 127
  message:
    bash: micromamba: command not found
bentsherman commented 1 year ago

I think there is still a bug here though:

https://github.com/nextflow-io/nextflow/blob/7d6ad62476bd43d6e98747cabe8bfe181d5da1e9/modules/nextflow/src/main/groovy/nextflow/executor/BashWrapperBuilder.groovy#L430-L437

The problem was never that the conda builder doesn't use micromamba, but that the bash wrapper uses conda info to activate the environment. If you happen to have conda installed it may work, but if not, I wouldn't expect it to work. This error would happen when the task is executed.

shenker commented 1 year ago

I can reproduce the two bugs discussed above: 1) --yes should be added to the micromamba create command. Otherwise one needs to manually set the envvar MAMBA_ALWAYS_YES=true when running nextflow. 2) The use of conda in the bash wrapper (as identified by @bentsherman above) needs to be replaced with micromamba. (A temporary workaround is to micromamba install conda, but nextflow should handle pure-micromamba installs, because pure-micromamba is probably the best practice for managing conda envs going forward.) To fix this, in line 434 of the above snippet, the conda/awk invocation can probably be replaced more simply by something like micromamba info|grep "env location :"|cut -d : -f 2.

Would be great to get a nextflow release that fixes these!

bentsherman commented 1 year ago

Thanks @shenker . Regarding the -y option, it looks like it is already added in most cases:

https://github.com/nextflow-io/nextflow/blob/89ede15c71e79858560f93df71064b74d2da2cf4/modules/nextflow/src/main/groovy/nextflow/conda/CondaCache.groovy#L284-L296

I think it is not added for conda env create because that command does not have a -y option. Is micromamba different?

shenker commented 1 year ago

Yes, micromamba env create accepts -y (it's confusing but the micromamba CLI is subtly different from the mamba/conda interfaces, the latter two being near-identical).

ewels commented 4 days ago

Should be solved in #4302 and released in 24.10.0, however the following error + log was reported in the nf-core Slack. Looks like Mamba is requesting confirmation for the mamba env create command?

Caused by:
  Failed to create Conda environment
    command: mamba env create --prefix /path/to/conda/environment/cache/directory/environment-9b0544c1a5024cf208947cbaff805d06 --file /root/.nextflow/assets/nf-core/rnaseq/./subworkflows/loc
al/prepare_genome/../../../modules/local/gtf_filter/environment.yml
    status : 143
    message:
      Transaction

        Prefix: /path/to/conda/environment/cache/directory/environment-9b0544c1a5024cf208947cbaff805d06

        Updating specs:

         - conda-forge::python=3.9.5

        Package               Version  Build               Channel          Size
      ────────────────────────────────────────────────────────────────────────────
        Install:
      ────────────────────────────────────────────────────────────────────────────

        + _libgcc_mutex           0.1  conda_forge         conda-forge       3kB
        + _openmp_mutex           4.5  2_gnu               conda-forge      24kB
        + ca-certificates   2024.8.30  hbcca054_0          conda-forge     159kB
        + ld_impl_linux-64       2.43  h712a8e2_2          conda-forge     669kB
        + libffi                  3.3  h58526e2_2          conda-forge      53kB
        + libgcc               14.2.0  h77fa898_1          conda-forge     849kB
        + libgcc-ng            14.2.0  h69a702a_1          conda-forge      54kB
        + libgomp              14.2.0  h77fa898_1          conda-forge     461kB
        + libsqlite            3.46.0  hde9e2c9_0          conda-forge     865kB
        + libstdcxx            14.2.0  hc0a3c3a_1          conda-forge       4MB
        + libstdcxx-ng         14.2.0  h4852527_1          conda-forge      54kB
        + libzlib              1.2.13  h4ab18f5_6          conda-forge      62kB
        + ncurses                 6.5  he02047a_1          conda-forge     889kB
        + openssl              1.1.1w  hd590300_0          conda-forge       2MB
        + pip                  24.3.1  pyh8b19718_0        conda-forge       1MB
        + python                3.9.5  h49503c6_0_cpython  conda-forge      29MB
        + readline                8.2  h8228510_1          conda-forge     281kB
        + setuptools           75.3.0  pyhd8ed1ab_0        conda-forge     780kB
        + sqlite               3.46.0  h6d4b2fc_0          conda-forge     860kB
        + tk                   8.6.13  noxft_h4845f30_101  conda-forge       3MB
        + tzdata                2024b  hc8b5060_0          conda-forge     122kB
        + wheel                0.44.0  pyhd8ed1ab_0        conda-forge      59kB
        + xz                    5.2.6  h166bdaf_0          conda-forge     418kB
        + zlib                 1.2.13  h4ab18f5_6          conda-forge      93kB
                                                                                                                                                                                     [293/695]
        Summary:

        Install: 24 packages

        Total download: 46MB

      ────────────────────────────────────────────────────────────────────────────

      Confirm changes: [Y/n]
Full output ``` nextflow run nf-core/rnaseq -r 3.17.0 -profile test,mamba --outdir lol [691/695] N E X T F L O W ~ version 24.10.0 Launching `https://github.com/nf-core/rnaseq` [pensive_meucci] DSL2 - revision: 00f924cf92 [3.17.0] WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ There is a problem with your Conda configuration! You will need to set-up the conda-forge and bioconda channels correctly. Please refer to https://bioconda.github.io/ The observed channel order is [defaults] but the following channel order is required: [conda-forge, bioconda] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" ------------------------------------------------------ ,--./,-. ___ __ __ __ ___ /,-._.--~' |\ | |__ __ / ` / \ |__) |__ } { | \| | \__, \__/ | \ |___ \`-._,-`-, `._,._,' nf-core/rnaseq 3.17.0 ------------------------------------------------------ Input/output options input : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/samplesheet/v3.10/samplesheet_test.csv outdir : lol Reference genome options fasta : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genome.fasta gtf : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes_with_empty_tid.gtf.gz gff : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes.gff.gz transcript_fasta : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/transcriptome.fasta additional_fasta : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/gfp.fa.gz hisat2_index : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/hisat2.tar.gz rsem_index : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/rsem.tar.gz salmon_index : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/salmon.tar.gz Read filtering options bbsplit_fasta_list : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/bbsplit_fasta_list.txt UMI options umitools_bc_pattern : NNNN [647/695] Alignment options pseudo_aligner : salmon min_mapped_reads : 5 Process skipping options skip_bbsplit : false Institutional config options config_profile_name : Test profile config_profile_description: Minimal test dataset to check pipeline function Core Nextflow options revision : 3.17.0 runName : pensive_meucci launchDir : /common/workdir workDir : /common/workdir/work projectDir : /root/.nextflow/assets/nf-core/rnaseq userName : root profile : test,mamba configFiles : !! Only displaying parameters that differ from the pipeline defaults !! ------------------------------------------------------ * The pipeline https://doi.org/10.5281/zenodo.1400710 * The nf-core framework https://doi.org/10.1038/s41587-020-0439-x * Software dependencies https://github.com/nf-core/rnaseq/blob/master/CITATIONS.md WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Both '--gtf' and '--gff' parameters have been provided. Using GTF file as priority. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ '--transcript_fasta' parameter has been provided. Make sure transcript names in this file match those in the GFF/GTF file. Please see: https://github.com/nf-core/rnaseq/issues/753 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ executor > local (7) [603/695] [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [ 0%] 0 of 1 [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - executor > local (7) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [ 0%] 0 of 1 [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - executor > local (7) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - executor > local (9) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - executor > local (9) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - executor > local (9) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - executor > local (9) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - executor > local (9) [558/695] [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - executor > local (9) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - executor > local (9) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - executor > local (9) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:STAR_GENOMEGENERATE - [ad/206a40] process > NFCORE_RNASEQ:PREPARE_GENOME:UNTAR_SALMON_INDEX (salmon.tar.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ - [f9/a40fd5] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP2) [100%] 3 of 3 [13/3dc6d3] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP2) [100%] 3 of 3 [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT - [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:FQ_SUBSAMPLE - [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:SALMON_QUANT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS - executor > local (9) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [513/695] [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:STAR_GENOMEGENERATE - [ad/206a40] process > NFCORE_RNASEQ:PREPARE_GENOME:UNTAR_SALMON_INDEX (salmon.tar.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ - [f9/a40fd5] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP2) [100%] 3 of 3 [13/3dc6d3] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP2) [100%] 3 of 3 [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT - [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:FQ_SUBSAMPLE - [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:SALMON_QUANT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:CUSTOM_TX2GENE - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:TXIMETA_TXIMPORT - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_LENGTH_SCALED - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_SCALED - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_TRANSCRIPT - [- ] process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS - [- ] process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE - [- ] process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS - [- ] process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_FW - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_REV - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDCLIP - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG - Plus 20 more processes waiting for tasks… [472/695] ERROR ~ Error executing process > 'NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER' Caused by: Failed to create Conda environment command: mamba env create --prefix /path/to/conda/environment/cache/directory/environment-9b0544c1a5024cf208947cbaff805d06 --file /root/.nextflow/assets/nf-core/rnaseq/./subworkflows/loc al/prepare_genome/../../../modules/local/gtf_filter/environment.yml status : 143 message: Transaction Prefix: /path/to/conda/environment/cache/directory/environment-9b0544c1a5024cf208947cbaff805d06 Updating specs: - conda-forge::python=3.9.5 Package Version Build Channel Size executor > local (9) [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:STAR_GENOMEGENERATE - [ad/206a40] process > NFCORE_RNASEQ:PREPARE_GENOME:UNTAR_SALMON_INDEX (salmon.tar.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ - [f9/a40fd5] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP2) [100%] 3 of 3 [13/3dc6d3] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP2) [100%] 3 of 3 [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT - [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:FQ_SUBSAMPLE - [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:SALMON_QUANT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT - [427/695] [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:CUSTOM_TX2GENE - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:TXIMETA_TXIMPORT - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_LENGTH_SCALED - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_SCALED - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_TRANSCRIPT - [- ] process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS - [- ] process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE - [- ] process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS - [- ] process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_FW - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_REV - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDCLIP - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG - Plus 20 more processes waiting for tasks… ERROR ~ Error executing process > 'NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER' Caused by: Failed to create Conda environment command: mamba env create --prefix /path/to/conda/environment/cache/directory/environment-9b0544c1a5024cf208947cbaff805d06 --file /root/.nextflow/assets/nf-core/rnaseq/./subworkflows/loc al/prepare_genome/../../../modules/local/gtf_filter/environment.yml status : 143 message: Transaction Prefix: /path/to/conda/environment/cache/directory/environment-9b0544c1a5024cf208947cbaff805d06 Updating specs: - conda-forge::python=3.9.5 Package Version Build Channel Size ──────────────────────────────────────────────────────────────────────────── Install: ──────────────────────────────────────────────────────────────────────────── + _libgcc_mutex 0.1 conda_forge conda-forge 3kB + _openmp_mutex 4.5 2_gnu conda-forge 24kB executor > local (9) [382/695] [99/441716] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER - [c9/2ef0c7] process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT - [- ] process > NFCORE_RNASEQ:PREPARE_GENOME:STAR_GENOMEGENERATE - [ad/206a40] process > NFCORE_RNASEQ:PREPARE_GENOME:UNTAR_SALMON_INDEX (salmon.tar.gz) [100%] 1 of 1 ✔ [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ - [f9/a40fd5] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP2) [100%] 3 of 3 [13/3dc6d3] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP2) [100%] 3 of 3 [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT - [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:FQ_SUBSAMPLE - [- ] process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:SALMON_QUANT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT - [- ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:CUSTOM_TX2GENE - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:TXIMETA_TXIMPORT - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_LENGTH_SCALED - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_SCALED - [- ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_TRANSCRIPT - [- ] process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT - [- ] process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS - [- ] process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE - [- ] process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS - [- ] process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_FW - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_REV - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDCLIP - [- ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG - Plus 20 more processes waiting for tasks… ERROR ~ Error executing process > 'NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER' [338/695] Caused by: Failed to create Conda environment command: mamba env create --prefix /path/to/conda/environment/cache/directory/environment-9b0544c1a5024cf208947cbaff805d06 --file /root/.nextflow/assets/nf-core/rnaseq/./subworkflows/loc al/prepare_genome/../../../modules/local/gtf_filter/environment.yml status : 143 message: Transaction Prefix: /path/to/conda/environment/cache/directory/environment-9b0544c1a5024cf208947cbaff805d06 Updating specs: - conda-forge::python=3.9.5 Package Version Build Channel Size ──────────────────────────────────────────────────────────────────────────── Install: ──────────────────────────────────────────────────────────────────────────── + _libgcc_mutex 0.1 conda_forge conda-forge 3kB + _openmp_mutex 4.5 2_gnu conda-forge 24kB + ca-certificates 2024.8.30 hbcca054_0 conda-forge 159kB + ld_impl_linux-64 2.43 h712a8e2_2 conda-forge 669kB + libffi 3.3 h58526e2_2 conda-forge 53kB + libgcc 14.2.0 h77fa898_1 conda-forge 849kB + libgcc-ng 14.2.0 h69a702a_1 conda-forge 54kB + libgomp 14.2.0 h77fa898_1 conda-forge 461kB + libsqlite 3.46.0 hde9e2c9_0 conda-forge 865kB + libstdcxx 14.2.0 hc0a3c3a_1 conda-forge 4MB + libstdcxx-ng 14.2.0 h4852527_1 conda-forge 54kB + libzlib 1.2.13 h4ab18f5_6 conda-forge 62kB + ncurses 6.5 he02047a_1 conda-forge 889kB + openssl 1.1.1w hd590300_0 conda-forge 2MB + pip 24.3.1 pyh8b19718_0 conda-forge 1MB + python 3.9.5 h49503c6_0_cpython conda-forge 29MB + readline 8.2 h8228510_1 conda-forge 281kB + setuptools 75.3.0 pyhd8ed1ab_0 conda-forge 780kB + sqlite 3.46.0 h6d4b2fc_0 conda-forge 860kB + tk 8.6.13 noxft_h4845f30_101 conda-forge 3MB + tzdata 2024b hc8b5060_0 conda-forge 122kB + wheel 0.44.0 pyhd8ed1ab_0 conda-forge 59kB + xz 5.2.6 h166bdaf_0 conda-forge 418kB + zlib 1.2.13 h4ab18f5_6 conda-forge 93kB [293/695] Summary: Install: 24 packages Total download: 46MB ──────────────────────────────────────────────────────────────────────────── Confirm changes: [Y/n] -- Check '.nextflow.log' file for details ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting -- Check '.nextflow.log' file for details ```
mamba version is 2.0.3
Rocky Linux 8.7 x86_64
jemma-nelson commented 2 days ago

You may be able to use the workaround described here: https://github.com/nextflow-io/nextflow/issues/4161#issuecomment-1709028041 - set export MAMBA_ALWAYS_YES=true in your shell environment. (if you are not using bash, the process for defining/exporting a environment variable may be different).