[Mar 25 07:14 PM]: OS: CentOS Linux 8, 104 cores, ~ 65 GB RAM. Python: 3.8.15
[Mar 25 07:14 PM]: Running funannotate v1.8.15
[Mar 25 07:14 PM]: Skipping CodingQuarry as no --rna_bam passed
[Mar 25 07:14 PM]: Parsed training data, run ab-initio gene predictors as follows:
Program Training-Method
augustus busco
genemark selftraining
glimmerhmm busco
snap busco
[Mar 25 07:14 PM]: Found 1 bad contigs, where alphabet is less than 4 [this should not happen]
[Mar 25 07:14 PM]: If you really want to keep and annotate these contigs (not recommended), pass --force
when I run "funannotate predict -i testsort.fa --species "Protomyces pachydermus" -o test --cpus 25", the error is:
[Mar 25 07:14 PM]: OS: CentOS Linux 8, 104 cores, ~ 65 GB RAM. Python: 3.8.15 [Mar 25 07:14 PM]: Running funanotate v1.8.15 [Mar 25 07:14 PM]: Soft-masking simple repeats with tantan [Mar 25 07:14 PM]: Repeat soft-masking finished: Masked genome: /opt/wgy_m25191434_898/GCA_003717275.1mark.fa num scaffolds: 329 assembly size: 12,007,681 bp masked repeats: 978,989 bp (8.15%)
[Mar 25 07:14 PM]: OS: CentOS Linux 8, 104 cores, ~ 65 GB RAM. Python: 3.8.15 [Mar 25 07:14 PM]: Running funannotate v1.8.15 [Mar 25 07:14 PM]: Skipping CodingQuarry as no --rna_bam passed [Mar 25 07:14 PM]: Parsed training data, run ab-initio gene predictors as follows: Program Training-Method augustus busco
genemark selftraining
glimmerhmm busco
snap busco
[Mar 25 07:14 PM]: Found 1 bad contigs, where alphabet is less than 4 [this should not happen] [Mar 25 07:14 PM]: If you really want to keep and annotate these contigs (not recommended), pass --force
so, what should I do?