I ran the funannotate train command with RNA-seq data downloaded from NCBI as follows:
sudo funannotate-docker train -i mask_sort_clean_isolate_contigs.fasta -o ./train -l FW.renamed.fastq.gz -r REV.renamed.fastq.gz --species "Trichoderma sp" --strain PKRF1 --cpus 35
The command gave an error as "CMD ERROR: stringtie -p 35 ./train/training/hisat2.coordSorted.bam", "Error creating output file ./tmp_nCC1al/stdout.08_07_14:20:48.tmp"
The funannotate test ran successfully without an error.
Please help
Hi,
I ran the funannotate train command with RNA-seq data downloaded from NCBI as follows: sudo funannotate-docker train -i mask_sort_clean_isolate_contigs.fasta -o ./train -l FW.renamed.fastq.gz -r REV.renamed.fastq.gz --species "Trichoderma sp" --strain PKRF1 --cpus 35 The command gave an error as "CMD ERROR: stringtie -p 35 ./train/training/hisat2.coordSorted.bam", "Error creating output file ./tmp_nCC1al/stdout.08_07_14:20:48.tmp"
The funannotate test ran successfully without an error. Please help